Developmental regulation of the expression of sodium currents in Xenopus primary neurons

The electrophysiological properties of neurons are determined by the expression of defined complements of ion channels. Nonetheless, the regulation mechanisms of the expression of neuronal ion channels are poorly understood, due in part to the diversity of neuron subtypes. We explored the expression...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	OLGUÍN,PATRICIO, ARMISEN,RICARDO, KUKULJAN,MANUEL
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	excitability REST/NRSF neuronal differentiation single-cell RT-PCR transcription
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300010
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602006000300010
record_format	dspace
spelling	oai:scielo:S0716-976020060003000102014-01-24Developmental regulation of the expression of sodium currents in Xenopus primary neuronsOLGUÍN,PATRICIOARMISEN,RICARDOKUKULJAN,MANUEL excitability REST/NRSF neuronal differentiation single-cell RT-PCR transcription The electrophysiological properties of neurons are determined by the expression of defined complements of ion channels. Nonetheless, the regulation mechanisms of the expression of neuronal ion channels are poorly understood, due in part to the diversity of neuron subtypes. We explored the expression of voltage-gated currents of Xenopus primary spinal neurons unequivocally identified by means of single-cell RT-PCR. We found that identified spinal neurons exhibit heterogeneity in the temporal appearance of voltage-gated currents. Nevertheless, all neurons progress to similar functional phenotypes. A physiological feature is the onset and increase of the expression of sodium currents. To understand the mechanisms underlying this process, we studied the effect of a dominant negative form of the transcriptional silencer REST/NRSF and found that it associates to an increase in the density of sodium currents. This observation is compatible with a role of this factor in the regulation of gene expression in neurons. These experiments constitute a proof of principle for the feasibility of analyzing molecular mechanisms of the regulation of ion channel genes during early neuronal development and provide direct evidence of the role of REST/NRSF in the control of neuronal sodium channel expression.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.3 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300010en10.4067/S0716-97602006000300010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	excitability REST/NRSF neuronal differentiation single-cell RT-PCR transcription
spellingShingle	excitability REST/NRSF neuronal differentiation single-cell RT-PCR transcription OLGUÍN,PATRICIO ARMISEN,RICARDO KUKULJAN,MANUEL Developmental regulation of the expression of sodium currents in Xenopus primary neurons
description	The electrophysiological properties of neurons are determined by the expression of defined complements of ion channels. Nonetheless, the regulation mechanisms of the expression of neuronal ion channels are poorly understood, due in part to the diversity of neuron subtypes. We explored the expression of voltage-gated currents of Xenopus primary spinal neurons unequivocally identified by means of single-cell RT-PCR. We found that identified spinal neurons exhibit heterogeneity in the temporal appearance of voltage-gated currents. Nevertheless, all neurons progress to similar functional phenotypes. A physiological feature is the onset and increase of the expression of sodium currents. To understand the mechanisms underlying this process, we studied the effect of a dominant negative form of the transcriptional silencer REST/NRSF and found that it associates to an increase in the density of sodium currents. This observation is compatible with a role of this factor in the regulation of gene expression in neurons. These experiments constitute a proof of principle for the feasibility of analyzing molecular mechanisms of the regulation of ion channel genes during early neuronal development and provide direct evidence of the role of REST/NRSF in the control of neuronal sodium channel expression.
author	OLGUÍN,PATRICIO ARMISEN,RICARDO KUKULJAN,MANUEL
author_facet	OLGUÍN,PATRICIO ARMISEN,RICARDO KUKULJAN,MANUEL
author_sort	OLGUÍN,PATRICIO
title	Developmental regulation of the expression of sodium currents in Xenopus primary neurons
title_short	Developmental regulation of the expression of sodium currents in Xenopus primary neurons
title_full	Developmental regulation of the expression of sodium currents in Xenopus primary neurons
title_fullStr	Developmental regulation of the expression of sodium currents in Xenopus primary neurons
title_full_unstemmed	Developmental regulation of the expression of sodium currents in Xenopus primary neurons
title_sort	developmental regulation of the expression of sodium currents in xenopus primary neurons
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300010
work_keys_str_mv	AT olguinpatricio developmentalregulationoftheexpressionofsodiumcurrentsinxenopusprimaryneurons AT armisenricardo developmentalregulationoftheexpressionofsodiumcurrentsinxenopusprimaryneurons AT kukuljanmanuel developmentalregulationoftheexpressionofsodiumcurrentsinxenopusprimaryneurons
_version_	1718441406068948992

Developmental regulation of the expression of sodium currents in Xenopus primary neurons

Ejemplares similares