Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line

Nuclear calcium appears to have an important role in the regulation of gene expression in many cells, but the mechanisms involved in controlling nuclear Ca2+ signaling are controversial and still poorly understood. We have described the presence of inositol 1,4,5 trisphosphate (IP3) receptors in the...

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Autores principales:	KUSNIER,CARLOS, CÁRDENAS,CÉSAR, HIDALGO,JORGE, JAIMOVICH,ENRIQUE
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	single channel cell nucleus IP3 receptor muscle
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300015
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spelling	oai:scielo:S0716-976020060003000152014-01-24Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell lineKUSNIER,CARLOSCÁRDENAS,CÉSARHIDALGO,JORGEJAIMOVICH,ENRIQUE single channel cell nucleus IP3 receptor muscle Nuclear calcium appears to have an important role in the regulation of gene expression in many cells, but the mechanisms involved in controlling nuclear Ca2+ signaling are controversial and still poorly understood. We have described the presence of inositol 1,4,5 trisphosphate (IP3) receptors in the nuclei of skeletal muscle cells. Now, we have characterized the properties of the IP3 receptors channels present in the nuclei of the 1B5 cell line, which do not express any isoforms of the ryanodine receptor. Immunocytochemistry of isolated nuclei confirmed the presence of IP3R in the nuclear envelope and fluorescence measurements in nuclei suspensions allowed us to document ATP-dependent calcium loading by the nucleus and its release upon IP3 addition. Patch clamp of nuclear membranes was performed, and single-channel activity recorded was dependent on the presence of IP3 in the pipette; single-channel conductance was in the range reported in the literature for these channels, and the open probability was shown to be dependent on IP3 concentration.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.3 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300015en10.4067/S0716-97602006000300015
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	single channel cell nucleus IP3 receptor muscle
spellingShingle	single channel cell nucleus IP3 receptor muscle KUSNIER,CARLOS CÁRDENAS,CÉSAR HIDALGO,JORGE JAIMOVICH,ENRIQUE Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
description	Nuclear calcium appears to have an important role in the regulation of gene expression in many cells, but the mechanisms involved in controlling nuclear Ca2+ signaling are controversial and still poorly understood. We have described the presence of inositol 1,4,5 trisphosphate (IP3) receptors in the nuclei of skeletal muscle cells. Now, we have characterized the properties of the IP3 receptors channels present in the nuclei of the 1B5 cell line, which do not express any isoforms of the ryanodine receptor. Immunocytochemistry of isolated nuclei confirmed the presence of IP3R in the nuclear envelope and fluorescence measurements in nuclei suspensions allowed us to document ATP-dependent calcium loading by the nucleus and its release upon IP3 addition. Patch clamp of nuclear membranes was performed, and single-channel activity recorded was dependent on the presence of IP3 in the pipette; single-channel conductance was in the range reported in the literature for these channels, and the open probability was shown to be dependent on IP3 concentration.
author	KUSNIER,CARLOS CÁRDENAS,CÉSAR HIDALGO,JORGE JAIMOVICH,ENRIQUE
author_facet	KUSNIER,CARLOS CÁRDENAS,CÉSAR HIDALGO,JORGE JAIMOVICH,ENRIQUE
author_sort	KUSNIER,CARLOS
title	Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
title_short	Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
title_full	Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
title_fullStr	Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
title_full_unstemmed	Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
title_sort	single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300015
work_keys_str_mv	AT kusniercarlos singlechannelrecordingofinositoltrisphosphatereceptorintheisolatednucleusofamusclecellline AT cardenascesar singlechannelrecordingofinositoltrisphosphatereceptorintheisolatednucleusofamusclecellline AT hidalgojorge singlechannelrecordingofinositoltrisphosphatereceptorintheisolatednucleusofamusclecellline AT jaimovichenrique singlechannelrecordingofinositoltrisphosphatereceptorintheisolatednucleusofamusclecellline
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Single-channel recording of inositol trisphosphate receptor in the isolated nucleus of a muscle cell line

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