Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets

During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic β-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insu...

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Autores principales:	CUNHA,DANIEL A, AMARAL,MARIA EC, CARVALHO,CAROLINA PF, COLLARES-BUZATO,CARLA B, CARNEIRO,EVERARDO M, BOSCHERO,ANTONIO C
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	insulin pancreatic islets pregnant rats prolactin SNARE proteins synaptotagmin
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016
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spelling	oai:scielo:S0716-976020060003000162014-01-24Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal isletsCUNHA,DANIEL AAMARAL,MARIA ECCARVALHO,CAROLINA PFCOLLARES-BUZATO,CARLA BCARNEIRO,EVERARDO MBOSCHERO,ANTONIO C insulin pancreatic islets pregnant rats prolactin SNARE proteins synaptotagmin During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic β-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and confocal microscopy. Transcription and expression of all SNARE genes and proteins were increased in islets from pregnant and PRL-treated neonatal rats when compared with controls. The only exception was VAMP-2 production in islets from pregnant rats. Increased mRNA and protein expression of synaptotagmin IV, but not the isoform I, also was observed in islets from pregnant and PRL-treated rats. This effect was not inhibited by wortmannin or PD098059, inhibitors of the PI3-kinase and MAPK pathways, respectively. As revealed by confocal laser microscopy, both syntaxin 1A and synaptotagmin IV were immunolocated in islet cells, including the insulin-containing cells. These results indicate that PRL modulates the final steps of insulin secretion by increasing the expression of proteins involved in membrane fusion.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.3 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016en10.4067/S0716-97602006000300016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	insulin pancreatic islets pregnant rats prolactin SNARE proteins synaptotagmin
spellingShingle	insulin pancreatic islets pregnant rats prolactin SNARE proteins synaptotagmin CUNHA,DANIEL A AMARAL,MARIA EC CARVALHO,CAROLINA PF COLLARES-BUZATO,CARLA B CARNEIRO,EVERARDO M BOSCHERO,ANTONIO C Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
description	During pregnancy and the perinatal period of life, prolactin (PRL) and other lactogenic substances induce adaptation and maturation of the stimulus-secretion coupling system in pancreatic β-cells. Since the SNARE molecules, SNAP-25, syntaxin 1, VAMP-2, and synaptotagmins participate in insulin secretion, we investigated whether the improved secretory response to glucose during these periods involves alteration in the expression of these proteins. mRNA was extracted from neonatal rat islets cultured for 5 days in the presence of PRL and from pregnant rats (17th-18th days of pregnancy) and reverse transcribed. The expression of genes was analyzed by semi-quantitative RT-PCR assay. The expression of proteins was analyzed by Western blotting and confocal microscopy. Transcription and expression of all SNARE genes and proteins were increased in islets from pregnant and PRL-treated neonatal rats when compared with controls. The only exception was VAMP-2 production in islets from pregnant rats. Increased mRNA and protein expression of synaptotagmin IV, but not the isoform I, also was observed in islets from pregnant and PRL-treated rats. This effect was not inhibited by wortmannin or PD098059, inhibitors of the PI3-kinase and MAPK pathways, respectively. As revealed by confocal laser microscopy, both syntaxin 1A and synaptotagmin IV were immunolocated in islet cells, including the insulin-containing cells. These results indicate that PRL modulates the final steps of insulin secretion by increasing the expression of proteins involved in membrane fusion.
author	CUNHA,DANIEL A AMARAL,MARIA EC CARVALHO,CAROLINA PF COLLARES-BUZATO,CARLA B CARNEIRO,EVERARDO M BOSCHERO,ANTONIO C
author_facet	CUNHA,DANIEL A AMARAL,MARIA EC CARVALHO,CAROLINA PF COLLARES-BUZATO,CARLA B CARNEIRO,EVERARDO M BOSCHERO,ANTONIO C
author_sort	CUNHA,DANIEL A
title	Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
title_short	Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
title_full	Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
title_fullStr	Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
title_full_unstemmed	Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets
title_sort	increased expression of snare proteins and synaptotagmin iv in islets from pregnant rats and in vitro prolactin-treated neonatal islets
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300016
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Increased expression of SNARE proteins and synaptotagmin IV in islets from pregnant rats and in vitro prolactin-treated neonatal islets

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