Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers

The effects of caffeine on the process of excitation-contraction coupling in amphibian skeletal muscle fibers were investigated using the confocal spot detection technique. This method permits to carefully discriminate between caffeine effects on the primary sources of Ca2+ release at the Z-lines wh...

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Autores principales:	VERGARA,JULIO L, DIFRANCO,MARINO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	excitation-contraction coupling caffeine confocal spot detection calcium transients frog skeletal muscle calcium microdomains
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300017
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spelling	oai:scielo:S0716-976020060003000172014-01-24Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibersVERGARA,JULIO LDIFRANCO,MARINO excitation-contraction coupling caffeine confocal spot detection calcium transients frog skeletal muscle calcium microdomains The effects of caffeine on the process of excitation-contraction coupling in amphibian skeletal muscle fibers were investigated using the confocal spot detection technique. This method permits to carefully discriminate between caffeine effects on the primary sources of Ca2+ release at the Z-lines where the triads are located and secondary actions on other potential Ca Release sources. Our results demonstrate that 0.5 mM caffeine potentiates and prolongs localized action-potential evoked Ca2+ transients recorded at the level of the Z-lines, but that 1mM only prolongs them. The effects at both doses are reversible. At the level of the M-line, localized Ca2+ transients displayed more variability in the presence of 1 mM caffeine than in control conditions. At this dose of caffeine, extra-junctional sources of Ca2+ release also were observed occasionally.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.3 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300017en10.4067/S0716-97602006000300017
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	excitation-contraction coupling caffeine confocal spot detection calcium transients frog skeletal muscle calcium microdomains
spellingShingle	excitation-contraction coupling caffeine confocal spot detection calcium transients frog skeletal muscle calcium microdomains VERGARA,JULIO L DIFRANCO,MARINO Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
description	The effects of caffeine on the process of excitation-contraction coupling in amphibian skeletal muscle fibers were investigated using the confocal spot detection technique. This method permits to carefully discriminate between caffeine effects on the primary sources of Ca2+ release at the Z-lines where the triads are located and secondary actions on other potential Ca Release sources. Our results demonstrate that 0.5 mM caffeine potentiates and prolongs localized action-potential evoked Ca2+ transients recorded at the level of the Z-lines, but that 1mM only prolongs them. The effects at both doses are reversible. At the level of the M-line, localized Ca2+ transients displayed more variability in the presence of 1 mM caffeine than in control conditions. At this dose of caffeine, extra-junctional sources of Ca2+ release also were observed occasionally.
author	VERGARA,JULIO L DIFRANCO,MARINO
author_facet	VERGARA,JULIO L DIFRANCO,MARINO
author_sort	VERGARA,JULIO L
title	Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
title_short	Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
title_full	Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
title_fullStr	Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
title_full_unstemmed	Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
title_sort	modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000300017
work_keys_str_mv	AT vergarajuliol modulationbycaffeineofcalciumreleasemicrodomainsinfrogskeletalmusclefibers AT difrancomarino modulationbycaffeineofcalciumreleasemicrodomainsinfrogskeletalmusclefibers
_version_	1718441407876694016

Modulation by caffeine of calcium-release microdomains in frog skeletal muscle fibers

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