Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium

In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding d...

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Autores principales: POLANCO,RUBÉN, CANESSA,PAULO, RIVAS,ALEXIS, LARRONDO,LUIS F, LOBOS,SERGIO, VICUÑA,RAFAEL
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2006
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007
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spelling oai:scielo:S0716-976020060005000072007-08-06Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporiumPOLANCO,RUBÉNCANESSA,PAULORIVAS,ALEXISLARRONDO,LUIS FLOBOS,SERGIOVICUÑA,RAFAEL Acel basidiomycete copper Phanerochaete chrysosporium In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetesinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.4 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007en10.4067/S0716-97602006000500007
institution Scielo Chile
collection Scielo Chile
language English
topic Acel
basidiomycete
copper
Phanerochaete chrysosporium
spellingShingle Acel
basidiomycete
copper
Phanerochaete chrysosporium
POLANCO,RUBÉN
CANESSA,PAULO
RIVAS,ALEXIS
LARRONDO,LUIS F
LOBOS,SERGIO
VICUÑA,RAFAEL
Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
description In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes
author POLANCO,RUBÉN
CANESSA,PAULO
RIVAS,ALEXIS
LARRONDO,LUIS F
LOBOS,SERGIO
VICUÑA,RAFAEL
author_facet POLANCO,RUBÉN
CANESSA,PAULO
RIVAS,ALEXIS
LARRONDO,LUIS F
LOBOS,SERGIO
VICUÑA,RAFAEL
author_sort POLANCO,RUBÉN
title Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
title_short Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
title_full Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
title_fullStr Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
title_full_unstemmed Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
title_sort cloning and functional characterization of the gene encoding the transcription factor acel in the basidiomycete phanerochaete chrysosporium
publisher Sociedad de Biología de Chile
publishDate 2006
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007
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