Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding d...
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| Lenguaje: | English |
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Sociedad de Biología de Chile
2006
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oai:scielo:S0716-976020060005000072007-08-06Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporiumPOLANCO,RUBÉNCANESSA,PAULORIVAS,ALEXISLARRONDO,LUIS FLOBOS,SERGIOVICUÑA,RAFAEL Acel basidiomycete copper Phanerochaete chrysosporium In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetesinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.4 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007en10.4067/S0716-97602006000500007 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Acel basidiomycete copper Phanerochaete chrysosporium |
| spellingShingle |
Acel basidiomycete copper Phanerochaete chrysosporium POLANCO,RUBÉN CANESSA,PAULO RIVAS,ALEXIS LARRONDO,LUIS F LOBOS,SERGIO VICUÑA,RAFAEL Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium |
| description |
In this report we describe the isolation and characterization of a gene encoding the transcription factor Acel (Activation protein of cup 1 Expression) in the white rot fungus Phanerochaete chrysosporium. Pc-acel encodes a predicted protein of 633 amino acids containing the copper-fist DNA binding domain typically found in fungal transcription factors such as Acel, Macl and Haal from Saccharomyces cerevisiae. The Pc-acel gene is localized in Scaffold 5, between coordinates 220841 and 222983. A S. cerevisiae acel null mutant strain unable to grow in high-copper medium was fully complemented by transformation with the cDNA of Pc-acel. Moreover, Northern blot hybridization studies indicated that Pc-acel cDNA restores copper inducibility of the yeast cup 1 gene, which encodes the metal-binding protein metallothionein implicated in copper resistance. To our knowledge, this is first report describing an Acel transcription factor in basidiomycetes |
| author |
POLANCO,RUBÉN CANESSA,PAULO RIVAS,ALEXIS LARRONDO,LUIS F LOBOS,SERGIO VICUÑA,RAFAEL |
| author_facet |
POLANCO,RUBÉN CANESSA,PAULO RIVAS,ALEXIS LARRONDO,LUIS F LOBOS,SERGIO VICUÑA,RAFAEL |
| author_sort |
POLANCO,RUBÉN |
| title |
Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium |
| title_short |
Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium |
| title_full |
Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium |
| title_fullStr |
Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium |
| title_full_unstemmed |
Cloning and functional characterization of the gene encoding the transcription factor Acel in the basidiomycete Phanerochaete chrysosporium |
| title_sort |
cloning and functional characterization of the gene encoding the transcription factor acel in the basidiomycete phanerochaete chrysosporium |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2006 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500007 |
| work_keys_str_mv |
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