Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein

Rotaviruses are the major cause of acute gastroenteritis in infants world-wide. The genome consists of eleven double stranded RNA segments. The major segment encodes the structural protein VP1, the viral RNA-dependent RNA polymerase (RdRp), which is a minor component of the viral inner core. This st...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	VÁSQUEZ-DEL CARPIÓ,RODRIGO, MORALES,JAIME L, BARRO,MARIO, RICARDO,ALBA, SPENCER,EUGENIO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	Rotavirus genome replication RNA polymerase bioinformatic analysis
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500008
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602006000500008
record_format	dspace
spelling	oai:scielo:S0716-976020060005000082007-08-06Bioinformatic prediction of polymerase elements in the rotavirus VP1 proteinVÁSQUEZ-DEL CARPIÓ,RODRIGOMORALES,JAIME LBARRO,MARIORICARDO,ALBASPENCER,EUGENIO Rotavirus genome replication RNA polymerase bioinformatic analysis Rotaviruses are the major cause of acute gastroenteritis in infants world-wide. The genome consists of eleven double stranded RNA segments. The major segment encodes the structural protein VP1, the viral RNA-dependent RNA polymerase (RdRp), which is a minor component of the viral inner core. This study is a detailed bioinformatic assessment of the VP1 sequence. Using various methods we have identified canonical motifs within the VP1 sequence which correspond to motifs previously identified within RdRps of other positive strand, double-strand RNA viruses. The study also predicts an overall structural conservation in the middle region that may correspond to the palm subdomain and part of the fingers and thumb subdomains, which comprise the polymerase core of the protein. Based on this analysis, we suggest that the rotavirus replicase has the minimal elements to function as an RNA-dependent RNA polymerase. VP1, besides having common RdRp features, also contains large unique regions that might be responsible for characteristic features observed in the Reoviridae familyinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.4 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500008en10.4067/S0716-97602006000500008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Rotavirus genome replication RNA polymerase bioinformatic analysis
spellingShingle	Rotavirus genome replication RNA polymerase bioinformatic analysis VÁSQUEZ-DEL CARPIÓ,RODRIGO MORALES,JAIME L BARRO,MARIO RICARDO,ALBA SPENCER,EUGENIO Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
description	Rotaviruses are the major cause of acute gastroenteritis in infants world-wide. The genome consists of eleven double stranded RNA segments. The major segment encodes the structural protein VP1, the viral RNA-dependent RNA polymerase (RdRp), which is a minor component of the viral inner core. This study is a detailed bioinformatic assessment of the VP1 sequence. Using various methods we have identified canonical motifs within the VP1 sequence which correspond to motifs previously identified within RdRps of other positive strand, double-strand RNA viruses. The study also predicts an overall structural conservation in the middle region that may correspond to the palm subdomain and part of the fingers and thumb subdomains, which comprise the polymerase core of the protein. Based on this analysis, we suggest that the rotavirus replicase has the minimal elements to function as an RNA-dependent RNA polymerase. VP1, besides having common RdRp features, also contains large unique regions that might be responsible for characteristic features observed in the Reoviridae family
author	VÁSQUEZ-DEL CARPIÓ,RODRIGO MORALES,JAIME L BARRO,MARIO RICARDO,ALBA SPENCER,EUGENIO
author_facet	VÁSQUEZ-DEL CARPIÓ,RODRIGO MORALES,JAIME L BARRO,MARIO RICARDO,ALBA SPENCER,EUGENIO
author_sort	VÁSQUEZ-DEL CARPIÓ,RODRIGO
title	Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
title_short	Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
title_full	Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
title_fullStr	Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
title_full_unstemmed	Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein
title_sort	bioinformatic prediction of polymerase elements in the rotavirus vp1 protein
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500008
work_keys_str_mv	AT vasquezdelcarpiorodrigo bioinformaticpredictionofpolymeraseelementsintherotavirusvp1protein AT moralesjaimel bioinformaticpredictionofpolymeraseelementsintherotavirusvp1protein AT barromario bioinformaticpredictionofpolymeraseelementsintherotavirusvp1protein AT ricardoalba bioinformaticpredictionofpolymeraseelementsintherotavirusvp1protein AT spencereugenio bioinformaticpredictionofpolymeraseelementsintherotavirusvp1protein
_version_	1718441410619768832

Bioinformatic prediction of polymerase elements in the rotavirus VP1 protein

Ejemplares similares