Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)

The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considere...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	CHARRIER,MAR YVONNE, FONTY,GERARD, GAILLARD-MARTINIE,BRIGITTE, AINOUCHE,KADER, ANDANT,GERARD
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2006
Materias:	Clostridium edible snails Enterobacteriaceae Enterococci gut microbiota Lactococci
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602006000500010
record_format	dspace
spelling	oai:scielo:S0716-976020060005000102007-08-06Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)CHARRIER,MAR YVONNEFONTY,GERARDGAILLARD-MARTINIE,BRIGITTEAINOUCHE,KADERANDANT,GERARD Clostridium edible snails Enterobacteriaceae Enterococci gut microbiota Lactococci The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g -1, but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared ≥ 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium, Raoultella and the Firmicutes with Enterococcus, Lactococcus, and Clostridium. According to the literature, these genera are mostly assigned to enteric environments or to phyllosphere, data in favour of culturing snails in contact with soil and plants. None of the strains were able to digest filter paper, Avicel cellulose or carboxymethyl cellulose (CMC). Acetogens and methanogenic archaea were not cultivated, so the fate of hydrogen remains questionable. This microbiota could play important roles in the digestive process (fermentation) and the energy supply of the snail (L-lactate, acetate). The choice of cereals and plants by snail farmers should take into account the fermentative abilities of the intestinal microbiotainfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.39 n.4 20062006-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010en10.4067/S0716-97602006000500010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Clostridium edible snails Enterobacteriaceae Enterococci gut microbiota Lactococci
spellingShingle	Clostridium edible snails Enterobacteriaceae Enterococci gut microbiota Lactococci CHARRIER,MAR YVONNE FONTY,GERARD GAILLARD-MARTINIE,BRIGITTE AINOUCHE,KADER ANDANT,GERARD Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
description	The intestinal microbiota of the edible snails Cornu aspersum fSyn: H. aspersa), and Helix pomatia were investigated by culture-based methods, 16S rRNA sequence analyses and phenotypic characterisations. The study was carried out on aestivating snails and two populations of H. pomatia were considered. The cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) CFU g -1, but the Swedish H. pomatia appeared significantly less colonised, suggesting a higher sensitivity of its microbiota to climatic change. All the strains, but one, shared ≥ 97% sequence identity with reference strains. They were arranged into two taxa: the Gamma Proteobacteria with Buttiauxella, Citrobacter, Enterobacter, Kluyvera, Obesumbacterium, Raoultella and the Firmicutes with Enterococcus, Lactococcus, and Clostridium. According to the literature, these genera are mostly assigned to enteric environments or to phyllosphere, data in favour of culturing snails in contact with soil and plants. None of the strains were able to digest filter paper, Avicel cellulose or carboxymethyl cellulose (CMC). Acetogens and methanogenic archaea were not cultivated, so the fate of hydrogen remains questionable. This microbiota could play important roles in the digestive process (fermentation) and the energy supply of the snail (L-lactate, acetate). The choice of cereals and plants by snail farmers should take into account the fermentative abilities of the intestinal microbiota
author	CHARRIER,MAR YVONNE FONTY,GERARD GAILLARD-MARTINIE,BRIGITTE AINOUCHE,KADER ANDANT,GERARD
author_facet	CHARRIER,MAR YVONNE FONTY,GERARD GAILLARD-MARTINIE,BRIGITTE AINOUCHE,KADER ANDANT,GERARD
author_sort	CHARRIER,MAR YVONNE
title	Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
title_short	Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
title_full	Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
title_fullStr	Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
title_full_unstemmed	Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)
title_sort	isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, helixpomatia and cornu aspersum (gastropoda: pulmonata)
publisher	Sociedad de Biología de Chile
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000500010
work_keys_str_mv	AT charriermaryvonne isolationandcharacterizationofcultivablefermentativebacteriafromtheintestineoftwoediblesnailshelixpomatiaandcornuaspersumgastropodapulmonata AT fontygerard isolationandcharacterizationofcultivablefermentativebacteriafromtheintestineoftwoediblesnailshelixpomatiaandcornuaspersumgastropodapulmonata AT gaillardmartiniebrigitte isolationandcharacterizationofcultivablefermentativebacteriafromtheintestineoftwoediblesnailshelixpomatiaandcornuaspersumgastropodapulmonata AT ainouchekader isolationandcharacterizationofcultivablefermentativebacteriafromtheintestineoftwoediblesnailshelixpomatiaandcornuaspersumgastropodapulmonata AT andantgerard isolationandcharacterizationofcultivablefermentativebacteriafromtheintestineoftwoediblesnailshelixpomatiaandcornuaspersumgastropodapulmonata
_version_	1718441411059122176

Isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, Helixpomatia and Cornu aspersum (Gastropoda: Pulmonata)

Ejemplares similares