Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii

The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the m...

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Autores principales:	LIU,BO, LIWANG,QI, RUIYANG,CHEN, SONG,WENQIN
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	combinatorial labeling probes fluorescence in situ hybridization karyotype Larix principis-rupprechtii LPD repeats 25S and 5S rDNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100003
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spelling	oai:scielo:S0716-976020070001000032007-11-15Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtiiLIU,BOLIWANG,QIRUIYANG,CHENSONG,WENQIN combinatorial labeling probes fluorescence in situ hybridization karyotype Larix principis-rupprechtii LPD repeats 25S and 5S rDNA The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the most extensively used methods in molecular cytogenetics producing chromosome landmarks, fluorescence in situ hybridization (FISH) has significantly facilitated karyotype construction, especially in species with morphologically similar chromosomes. This study developed a simple but effective use of combinatorial labeling probes to distinguish chromosomes of Larix principis-rupprechtii by multicolor FISH. Three highly repetitive sequences in Larix were selected: 25S rDNA hybridized at all of the secondary constrictions of two pairs of metacentrics and the largest pair of submetacentrics; 5S rDNA hybridized at subtelomeric sites of one pair of metacentrics that also harboured 25S rDNA on different arms; LPD family sequences are tandem repeats hybridized at proximal regions of 22 chromosomes. The three different probes were labeled with only two different labels, hybridized to metaphase chromosomes of Larix principis-rupprechtii, simultaneously visualized, and unequivocally distinguished in a single FISH experiment. These multicolor FISH marks largely improved the karyotype analysis of Larix principis-rupprechtiiinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100003en10.4067/S0716-97602007000100003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	combinatorial labeling probes fluorescence in situ hybridization karyotype Larix principis-rupprechtii LPD repeats 25S and 5S rDNA
spellingShingle	combinatorial labeling probes fluorescence in situ hybridization karyotype Larix principis-rupprechtii LPD repeats 25S and 5S rDNA LIU,BO LIWANG,QI RUIYANG,CHEN SONG,WENQIN Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
description	The chromosomes (2n = 2x = 24) of Larix principis-rupprechtii are composed of six pairs of large metacentrics and six pairs of medium-sized submetacentrics. The identification of homologous pairs is hampered by their high degree of similarity at the morphological level in each group. As one of the most extensively used methods in molecular cytogenetics producing chromosome landmarks, fluorescence in situ hybridization (FISH) has significantly facilitated karyotype construction, especially in species with morphologically similar chromosomes. This study developed a simple but effective use of combinatorial labeling probes to distinguish chromosomes of Larix principis-rupprechtii by multicolor FISH. Three highly repetitive sequences in Larix were selected: 25S rDNA hybridized at all of the secondary constrictions of two pairs of metacentrics and the largest pair of submetacentrics; 5S rDNA hybridized at subtelomeric sites of one pair of metacentrics that also harboured 25S rDNA on different arms; LPD family sequences are tandem repeats hybridized at proximal regions of 22 chromosomes. The three different probes were labeled with only two different labels, hybridized to metaphase chromosomes of Larix principis-rupprechtii, simultaneously visualized, and unequivocally distinguished in a single FISH experiment. These multicolor FISH marks largely improved the karyotype analysis of Larix principis-rupprechtii
author	LIU,BO LIWANG,QI RUIYANG,CHEN SONG,WENQIN
author_facet	LIU,BO LIWANG,QI RUIYANG,CHEN SONG,WENQIN
author_sort	LIU,BO
title	Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
title_short	Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
title_full	Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
title_fullStr	Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
title_full_unstemmed	Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii
title_sort	multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of larix principis-rupprechtii
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100003
work_keys_str_mv	AT liubo multicolorfluorescenceinsituhybridizationwithcombinatoriallabelingprobesenablesadetailedkaryotypeanalysisoflarixprincipisrupprechtii AT liwangqi multicolorfluorescenceinsituhybridizationwithcombinatoriallabelingprobesenablesadetailedkaryotypeanalysisoflarixprincipisrupprechtii AT ruiyangchen multicolorfluorescenceinsituhybridizationwithcombinatoriallabelingprobesenablesadetailedkaryotypeanalysisoflarixprincipisrupprechtii AT songwenqin multicolorfluorescenceinsituhybridizationwithcombinatoriallabelingprobesenablesadetailedkaryotypeanalysisoflarixprincipisrupprechtii
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Multicolor fluorescence in situ hybridization with combinatorial labeling probes enables a detailed karyotype analysis of Larix principis-rupprechtii

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