Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification?

In observations by confocal or conventional fluorescence microscopy, important factors should be considered in order to obtain accurate images. One of them, such as the fluorescence bleaching from highest intensity to lowest signal of fluorescence is a common problem with several DNA fluorochromes a...

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Autores principales: GALLARDO-ESCÁRATE,CRISTIAN, ÁLVAREZ-BORREGO,JOSUÉ, VON BRAND,ELISABETH, DUPRÉ,ENRIQUE, DEL RÍO-PORTILLA,MIGUEL ÁNGEL
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2007
Materias:
Nuclear DNA content
fluorescence fading
image analysis
DAPI
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100004
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