Asexual Recombination in a uvsH Mutant of Aspergillus nidulans

Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of ase...

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Autores principales:	SOUZA-JÚNIOR,S A, BECKER,T C A, CASTRO-PRADO,M A A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	mitotic crossing-over parameiosis uvs mutation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100007
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spelling	oai:scielo:S0716-976020070001000072007-11-15Asexual Recombination in a uvsH Mutant of Aspergillus nidulansSOUZA-JÚNIOR,S ABECKER,T C ACASTRO-PRADO,M A A mitotic crossing-over parameiosis uvs mutation Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of asexual recombinants (parameiotic segregants). The mutant uvsH, named B511, showed normal frequency of meiotic recombination in sexual crosses and high frequency of parameiotic segregants in the parasexual crossings with master strains (B511//A757 and B511//A288). Asexual haploid recombinants (parameiotic segregants), diploid and aneuploid segregants were recovered directly from the uvs//uvs+ heterokaryons (B511//A757 and B511// A288). Parameiotic segregants originated through mitotic crossing-over and independent assortment of chromosomesinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100007en10.4067/S0716-97602007000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	mitotic crossing-over parameiosis uvs mutation
spellingShingle	mitotic crossing-over parameiosis uvs mutation SOUZA-JÚNIOR,S A BECKER,T C A CASTRO-PRADO,M A A Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
description	Mutations in the gene uvsH of Aspergillus nidulans result in increased spontaneous chromosome instability and increased intragenic and intergenic mitotic recombination in homozygous diploids. The aim of the present work was to obtain a uvs mutant of A. nidulans and to use it for the isolation of asexual recombinants (parameiotic segregants). The mutant uvsH, named B511, showed normal frequency of meiotic recombination in sexual crosses and high frequency of parameiotic segregants in the parasexual crossings with master strains (B511//A757 and B511//A288). Asexual haploid recombinants (parameiotic segregants), diploid and aneuploid segregants were recovered directly from the uvs//uvs+ heterokaryons (B511//A757 and B511// A288). Parameiotic segregants originated through mitotic crossing-over and independent assortment of chromosomes
author	SOUZA-JÚNIOR,S A BECKER,T C A CASTRO-PRADO,M A A
author_facet	SOUZA-JÚNIOR,S A BECKER,T C A CASTRO-PRADO,M A A
author_sort	SOUZA-JÚNIOR,S A
title	Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
title_short	Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
title_full	Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
title_fullStr	Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
title_full_unstemmed	Asexual Recombination in a uvsH Mutant of Aspergillus nidulans
title_sort	asexual recombination in a uvsh mutant of aspergillus nidulans
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100007
work_keys_str_mv	AT souzajuniorsa asexualrecombinationinauvshmutantofaspergillusnidulans AT beckertca asexualrecombinationinauvshmutantofaspergillusnidulans AT castropradomaa asexualrecombinationinauvshmutantofaspergillusnidulans
_version_	1718441413247500288

Asexual Recombination in a uvsH Mutant of Aspergillus nidulans

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