Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous

In the yeast Xanthophyllomyces dendrorhous the genes idi, crtE, crtYB, crtl and ast are involved in the biosynthesis of astaxanthin from isopentenyl pyrophosphate. The carotenoid production and the kinetics of mRNA expression of structural genes controlling the carotenogenesis in a wild-type ATCC 24...

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Autores principales:	LODATO,P, ALCAÍNO,J, BARAHONA,S, NIKLITSCHEK,M, CARMONA,M, WOZNIAK,A, BAEZA,M, JIMÉNEZ,A, CIFUENTES,V
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	Xanthophyllomyces dendrorhous carotenogenesis genes mRNA expression
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100008
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spelling	oai:scielo:S0716-976020070001000082007-11-15Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhousLODATO,PALCAÍNO,JBARAHONA,SNIKLITSCHEK,MCARMONA,MWOZNIAK,ABAEZA,MJIMÉNEZ,ACIFUENTES,V Xanthophyllomyces dendrorhous carotenogenesis genes mRNA expression In the yeast Xanthophyllomyces dendrorhous the genes idi, crtE, crtYB, crtl and ast are involved in the biosynthesis of astaxanthin from isopentenyl pyrophosphate. The carotenoid production and the kinetics of mRNA expression of structural genes controlling the carotenogenesis in a wild-type ATCC 24230 and in carotenoid overproducer deregulated atxS2 strains were studied. The biosynthesis of carotenoid was induced at the late exponential growth phase in both strains. However, the cellular carotenoid concentration was four times higher in atxS2 than in the wild-type strain in the exponential growth phase, suggesting that carotenogenesis was deregulated in atxS2 at the beginning of growth. In addition, the maximum expression of the carotenogenesis genes at the mRNA level was observed during the induction period of carotenoid biosynthesis in the wild-type strain. The mRNA level of the crtYB, crtl, ast genes and to a lesser extent the idi gene, decayed at the end of the exponential growth phase. The mRNA levels of the crtE gene remained high along the whole growth curve of the yeast. In the atxS2 strain the mRNA levels of crtE gene were about two times higher than the wild-type strain in the early phase of the growth cycleinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100008en10.4067/S0716-97602007000100008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Xanthophyllomyces dendrorhous carotenogenesis genes mRNA expression
spellingShingle	Xanthophyllomyces dendrorhous carotenogenesis genes mRNA expression LODATO,P ALCAÍNO,J BARAHONA,S NIKLITSCHEK,M CARMONA,M WOZNIAK,A BAEZA,M JIMÉNEZ,A CIFUENTES,V Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
description	In the yeast Xanthophyllomyces dendrorhous the genes idi, crtE, crtYB, crtl and ast are involved in the biosynthesis of astaxanthin from isopentenyl pyrophosphate. The carotenoid production and the kinetics of mRNA expression of structural genes controlling the carotenogenesis in a wild-type ATCC 24230 and in carotenoid overproducer deregulated atxS2 strains were studied. The biosynthesis of carotenoid was induced at the late exponential growth phase in both strains. However, the cellular carotenoid concentration was four times higher in atxS2 than in the wild-type strain in the exponential growth phase, suggesting that carotenogenesis was deregulated in atxS2 at the beginning of growth. In addition, the maximum expression of the carotenogenesis genes at the mRNA level was observed during the induction period of carotenoid biosynthesis in the wild-type strain. The mRNA level of the crtYB, crtl, ast genes and to a lesser extent the idi gene, decayed at the end of the exponential growth phase. The mRNA levels of the crtE gene remained high along the whole growth curve of the yeast. In the atxS2 strain the mRNA levels of crtE gene were about two times higher than the wild-type strain in the early phase of the growth cycle
author	LODATO,P ALCAÍNO,J BARAHONA,S NIKLITSCHEK,M CARMONA,M WOZNIAK,A BAEZA,M JIMÉNEZ,A CIFUENTES,V
author_facet	LODATO,P ALCAÍNO,J BARAHONA,S NIKLITSCHEK,M CARMONA,M WOZNIAK,A BAEZA,M JIMÉNEZ,A CIFUENTES,V
author_sort	LODATO,P
title	Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
title_short	Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
title_full	Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
title_fullStr	Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
title_full_unstemmed	Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous
title_sort	expression of the carotenoid biosynthesis genes in xanthophyllomyces dendrorhous
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100008
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Expression of the carotenoid biosynthesis genes in Xanthophyllomyces dendrorhous

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