Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida

This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp...

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Autores principales:	MANCUSO,MONIQUE, AVENDAÑO-HERRERA,RUBÉN, ZACCONE,R, TORANZO,ALICIA E, MAGARIÑOS,BEATRIZ
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	Photobacterium damselae ssp piscicida RAPD ERIC-PCR REP-PCR
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100009
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spelling	oai:scielo:S0716-976020070001000092007-11-15Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicidaMANCUSO,MONIQUEAVENDAÑO-HERRERA,RUBÉNZACCONE,RTORANZO,ALICIA EMAGARIÑOS,BEATRIZ Photobacterium damselae ssp piscicida RAPD ERIC-PCR REP-PCR This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infectionsinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100009en10.4067/S0716-97602007000100009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Photobacterium damselae ssp piscicida RAPD ERIC-PCR REP-PCR
spellingShingle	Photobacterium damselae ssp piscicida RAPD ERIC-PCR REP-PCR MANCUSO,MONIQUE AVENDAÑO-HERRERA,RUBÉN ZACCONE,R TORANZO,ALICIA E MAGARIÑOS,BEATRIZ Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
description	This study evaluates the effectiveness of three different molecular techniques, repetitive extragenic palindromic PCR (REP-PCR), enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and the random amplified polymorphic DNA (RAPD-PCR) for rapid typing of Photobacterium damselae ssp. piscicida strains isolated from different species of marine fish and geographic areas. The results obtained by the three methods showed that RAPD and ERIC-PCR were more discriminative for suitable rapid typing of Ph. damselae ssp. piscicida than REP-PCR. The analysis of DNA banding patterns generated by both molecular methods (RAPD and ERIC-PCR) clearly separated the strains into two main groups that strongly correlated with their geographic origin. Moreover, the REP-PCR analysis was less reproducible than the RAPD and ERIC-PCR methods and does not allow the establishment of genetic groups. RAPD and ERIC-PCR constitute valuable tools for molecular typing of Ph. damselae ssp. piscicida strains, which can be used in epidemiological studies of photobacteriosis infections
author	MANCUSO,MONIQUE AVENDAÑO-HERRERA,RUBÉN ZACCONE,R TORANZO,ALICIA E MAGARIÑOS,BEATRIZ
author_facet	MANCUSO,MONIQUE AVENDAÑO-HERRERA,RUBÉN ZACCONE,R TORANZO,ALICIA E MAGARIÑOS,BEATRIZ
author_sort	MANCUSO,MONIQUE
title	Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
title_short	Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
title_full	Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
title_fullStr	Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
title_full_unstemmed	Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida
title_sort	evaluation of different dna-based fingerprinting methods for typing photobacterium damselae ssp. piscicida
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000100009
work_keys_str_mv	AT mancusomonique evaluationofdifferentdnabasedfingerprintingmethodsfortypingphotobacteriumdamselaessppiscicida AT avendanoherreraruben evaluationofdifferentdnabasedfingerprintingmethodsfortypingphotobacteriumdamselaessppiscicida AT zacconer evaluationofdifferentdnabasedfingerprintingmethodsfortypingphotobacteriumdamselaessppiscicida AT toranzoaliciae evaluationofdifferentdnabasedfingerprintingmethodsfortypingphotobacteriumdamselaessppiscicida AT magarinosbeatriz evaluationofdifferentdnabasedfingerprintingmethodsfortypingphotobacteriumdamselaessppiscicida
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Evaluation of different DNA-based fingerprinting methods for typing Photobacterium damselae ssp. piscicida

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