Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress

We quantified the ozone impact on levels of Zea mays L. cv. Chambord mRNAs encoding C4-phosphoenolpyruvate carboxylase (C4-PEPc), ribulose-l,5-bisphosphate carboxylase/oxygenase small and large subunits (Rubisco-SSU and Rubisco-LSU, respectively) and Rubisco activase (RCA) using real-time RT-PCR. Fo...

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Autores principales:	LEITAO,LOUIS, MAORET,JEAN-JOSÉ, BIOLLEY,JEAN-PHILIPPE
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	carboxylase maize mRNA ozone Rubisco activase
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200005
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spelling	oai:scielo:S0716-976020070002000052008-06-13Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stressLEITAO,LOUISMAORET,JEAN-JOSÉBIOLLEY,JEAN-PHILIPPE carboxylase maize mRNA ozone Rubisco activase We quantified the ozone impact on levels of Zea mays L. cv. Chambord mRNAs encoding C4-phosphoenolpyruvate carboxylase (C4-PEPc), ribulose-l,5-bisphosphate carboxylase/oxygenase small and large subunits (Rubisco-SSU and Rubisco-LSU, respectively) and Rubisco activase (RCA) using real-time RT-PCR. Foliar pigment content, PEPc and Rubisco protein amounts were simultaneously determined. Two experiments were performed to study the ozone response of the 5th and the 10th leaf. For each experiment, three ozone concentrations were tested in open-top chambers: non-filtered air (NF, control) and non-filtered air containing 40 (+40) and 80 nL L-1 (+80) ozone. Regarding the 5th leaf, +40 atmosphere induced a loss in pigmentation, PEPc and Rubisco activase mRNAs. However, it was unable to notably depress carboxylase protein amounts and mRNAs encoding Rubisco. Except for Rubisco mRNAs, all other measured parameters from 5th leaf were depressed by +80 atmosphere. Regarding the 10th leaf, +40 atmosphere increased photosynthetic pigments and transcripts encoding Rubisco and Rubisco activase. Rubisco and PEPc protein amounts were not drastically changed, even if they tended to be increased. Level of C4-PEPc mRNA remained almost stable. In response to +80 atmosphere, pigments and transcripts encoding PEPc were notably decreased. Rubisco and PEPc protein amounts also declined to a lesser extent. Conversely, the level of transcripts encoding both Rubisco subunits and Rubisco activase that were not consistently disturbed tended to be slightly augmented. So, the present study suggests that maize leaves can respond differentially to a similar ozone stressinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.2 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200005en10.4067/S0716-97602007000200005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	carboxylase maize mRNA ozone Rubisco activase
spellingShingle	carboxylase maize mRNA ozone Rubisco activase LEITAO,LOUIS MAORET,JEAN-JOSÉ BIOLLEY,JEAN-PHILIPPE Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
description	We quantified the ozone impact on levels of Zea mays L. cv. Chambord mRNAs encoding C4-phosphoenolpyruvate carboxylase (C4-PEPc), ribulose-l,5-bisphosphate carboxylase/oxygenase small and large subunits (Rubisco-SSU and Rubisco-LSU, respectively) and Rubisco activase (RCA) using real-time RT-PCR. Foliar pigment content, PEPc and Rubisco protein amounts were simultaneously determined. Two experiments were performed to study the ozone response of the 5th and the 10th leaf. For each experiment, three ozone concentrations were tested in open-top chambers: non-filtered air (NF, control) and non-filtered air containing 40 (+40) and 80 nL L-1 (+80) ozone. Regarding the 5th leaf, +40 atmosphere induced a loss in pigmentation, PEPc and Rubisco activase mRNAs. However, it was unable to notably depress carboxylase protein amounts and mRNAs encoding Rubisco. Except for Rubisco mRNAs, all other measured parameters from 5th leaf were depressed by +80 atmosphere. Regarding the 10th leaf, +40 atmosphere increased photosynthetic pigments and transcripts encoding Rubisco and Rubisco activase. Rubisco and PEPc protein amounts were not drastically changed, even if they tended to be increased. Level of C4-PEPc mRNA remained almost stable. In response to +80 atmosphere, pigments and transcripts encoding PEPc were notably decreased. Rubisco and PEPc protein amounts also declined to a lesser extent. Conversely, the level of transcripts encoding both Rubisco subunits and Rubisco activase that were not consistently disturbed tended to be slightly augmented. So, the present study suggests that maize leaves can respond differentially to a similar ozone stress
author	LEITAO,LOUIS MAORET,JEAN-JOSÉ BIOLLEY,JEAN-PHILIPPE
author_facet	LEITAO,LOUIS MAORET,JEAN-JOSÉ BIOLLEY,JEAN-PHILIPPE
author_sort	LEITAO,LOUIS
title	Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
title_short	Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
title_full	Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
title_fullStr	Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
title_full_unstemmed	Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress
title_sort	changes in pep carboxylase, rubisco and rubisco activase mrna levels from maize (zea mays) exposed to a chronic ozone stress
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200005
work_keys_str_mv	AT leitaolouis changesinpepcarboxylaserubiscoandrubiscoactivasemrnalevelsfrommaizezeamaysexposedtoachronicozonestress AT maoretjeanjose changesinpepcarboxylaserubiscoandrubiscoactivasemrnalevelsfrommaizezeamaysexposedtoachronicozonestress AT biolleyjeanphilippe changesinpepcarboxylaserubiscoandrubiscoactivasemrnalevelsfrommaizezeamaysexposedtoachronicozonestress
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Changes in PEP Carboxylase, Rubisco and Rubisco activase mRNA levels from maize (Zea mays) exposed to a chronic ozone stress

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