Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin

Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant tox...

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Autores principales:	AVILA,ANA D, CALDERÓN,CARLOS F, PEREZ,RITA M, PONS,CARMEN, PEREDA,CELIA M, ORTIZ,ANA R
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	Epidermal growth factor epidermal growth factor receptor hemolytic toxins immunotoxins monoclonal antibodies
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008
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spelling	oai:scielo:S0716-976020070002000082008-06-13Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxinAVILA,ANA DCALDERÓN,CARLOS FPEREZ,RITA MPONS,CARMENPEREDA,CELIA MORTIZ,ANA R Epidermal growth factor epidermal growth factor receptor hemolytic toxins immunotoxins monoclonal antibodies Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that express the human epidermal growth factor receptor, but non-toxic for U1906 cells that do not express this receptorinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.2 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008en10.4067/S0716-97602007000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Epidermal growth factor epidermal growth factor receptor hemolytic toxins immunotoxins monoclonal antibodies
spellingShingle	Epidermal growth factor epidermal growth factor receptor hemolytic toxins immunotoxins monoclonal antibodies AVILA,ANA D CALDERÓN,CARLOS F PEREZ,RITA M PONS,CARMEN PEREDA,CELIA M ORTIZ,ANA R Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
description	Hybrid molecules obtained through conjugation of monoclonal antibodies and toxins constitute an approach under exploration to generate potential agents for the treatment of cancer and other diseases. A frequently employed toxic component in the construction of such immunotoxins is ricin, a plant toxin which inhibits protein synthesis at ribosomal level and so requires to be internalized by the cell. A hemolytic toxin isolated from the sea anemone Stichodactyla helianthus, which is active at the cell membrane level, was linked through a disulfide bond to the anti-epidermal growth factor receptor monoclonal antibody ior egf/r3. The resulting immunotoxin did not exhibit hemolytic activity except under reducing conditions. It was toxic for H125 cells that express the human epidermal growth factor receptor, but non-toxic for U1906 cells that do not express this receptor
author	AVILA,ANA D CALDERÓN,CARLOS F PEREZ,RITA M PONS,CARMEN PEREDA,CELIA M ORTIZ,ANA R
author_facet	AVILA,ANA D CALDERÓN,CARLOS F PEREZ,RITA M PONS,CARMEN PEREDA,CELIA M ORTIZ,ANA R
author_sort	AVILA,ANA D
title	Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
title_short	Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
title_full	Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
title_fullStr	Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
title_full_unstemmed	Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
title_sort	construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200008
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Construction of an immunotoxin by linking a monoclonal antibody against the human epidermal growth factor receptor and a hemolytic toxin

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