The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle

We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the...

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Autores principales:	ZIMMERMANN,KATRIN, HOLTZ,MARLIS, PHI-VAN,LOC
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	chromatin structure origin of bidirectional replication Lysozyme GAS41 promoter
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009
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spelling	oai:scielo:S0716-976020070002000092008-06-13The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycleZIMMERMANN,KATRINHOLTZ,MARLISPHI-VAN,LOC chromatin structure origin of bidirectional replication Lysozyme GAS41 promoter We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phaseinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.2 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009en10.4067/S0716-97602007000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	chromatin structure origin of bidirectional replication Lysozyme GAS41 promoter
spellingShingle	chromatin structure origin of bidirectional replication Lysozyme GAS41 promoter ZIMMERMANN,KATRIN HOLTZ,MARLIS PHI-VAN,LOC The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
description	We used a rapid and simple protocol using lysolecithin for mapping HS sites in vivo. The protocol is based on partial digestion with DNase I of exponentially growing cells following permeabilization by short treatment with lysolecithin. Using this protocol, we analyzed the chromatin structure of the region surrounding two overlapping elements, an origin of bidirectional DNA replication and the GAS41 promoter, in chicken myelomonocytic HD11 cells arrested in G0, G0 and S phases as well as at the G0/S border. The results show that the chromatin of this region became more nuclease sensitive when cells were arrested in G0 phase and that this change in chromatin structure was reversible after the cells began to enter S phase
author	ZIMMERMANN,KATRIN HOLTZ,MARLIS PHI-VAN,LOC
author_facet	ZIMMERMANN,KATRIN HOLTZ,MARLIS PHI-VAN,LOC
author_sort	ZIMMERMANN,KATRIN
title	The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
title_short	The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
title_full	The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
title_fullStr	The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
title_full_unstemmed	The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle
title_sort	chromatin structure of the lysozyme gas41 origin of dna replication changes during the cell cycle
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200009
work_keys_str_mv	AT zimmermannkatrin thechromatinstructureofthelysozymegas41originofdnareplicationchangesduringthecellcycle AT holtzmarlis thechromatinstructureofthelysozymegas41originofdnareplicationchangesduringthecellcycle AT phivanloc thechromatinstructureofthelysozymegas41originofdnareplicationchangesduringthecellcycle AT zimmermannkatrin chromatinstructureofthelysozymegas41originofdnareplicationchangesduringthecellcycle AT holtzmarlis chromatinstructureofthelysozymegas41originofdnareplicationchangesduringthecellcycle AT phivanloc chromatinstructureofthelysozymegas41originofdnareplicationchangesduringthecellcycle
_version_	1718441416091238400

The chromatin structure of the lysozyme GAS41 origin of DNA replication changes during the cell cycle

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