Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells
Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possib...
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Sociedad de Biología de Chile
2007
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oai:scielo:S0716-976020070002000142008-06-13Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cellsTORO,ANDRÉSARREDONDO,CRISTIANCORDOVA,GONZALOARAYA,CLAUDIAPALACIOS,JOSÉ LVENEGAS,ALEJANDROMORITA,MASASHIIMANAKA,TSUNEOSANTOS,MANUEL J adrenoleukodystrophy ALDRP endoplasmic reticulum peroxisome biogenesis Pex3p and PMP70 Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The expressed proteins were targeted to peroxisomes and peroxisomal ghosts correctly in the presence or absence of Brefeldin A (BFA), a drug known to block the ER-Golgi transit. Furthermore, low temperature did not disturb the targeting of Pex3p-GFP to peroxisomes. We also constructed two chimeric proteins of PMPs containing an ER retention signal "DEKKMP": GFP-ALDRP-DEKKMP and myc- Pex3p-DEKKMP. These proteins were mostly targeted to peroxisomes. No colocalization with an ER maker was found. These results suggest that the classical ER-Golgi pathway does not play a major role in the biogenesis of mammalian PMPsinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.2 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200014en10.4067/S0716-97602007000200014 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
adrenoleukodystrophy ALDRP endoplasmic reticulum peroxisome biogenesis Pex3p and PMP70 |
| spellingShingle |
adrenoleukodystrophy ALDRP endoplasmic reticulum peroxisome biogenesis Pex3p and PMP70 TORO,ANDRÉS ARREDONDO,CRISTIAN CORDOVA,GONZALO ARAYA,CLAUDIA PALACIOS,JOSÉ L VENEGAS,ALEJANDRO MORITA,MASASHI IMANAKA,TSUNEO SANTOS,MANUEL J Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells |
| description |
Peroxisomes are thought to be formed by division of pre-existing peroxisomes after the import of newly synthesized proteins. However, it has been recently suggested that the endoplasmic reticulum (ER) provides an alternative de novo mechanism for peroxisome biogenesis in some cells. To test a possible role of the ER-Golgi transit in peroxisome biogenesis in mammalian cells, we evaluated the biogenesis of three peroxisomal membrane proteins (PMPs): ALDRP (adrenoleukodystrophy related protein), PMP70 and Pex3p in CHO cells. We constructed chimeric genes encoding these PMPs and green fluorescent protein (GFP), and transiently transfected them to wild type and mutant CHO cells, in which normal peroxisomes were replaced by peroxisomal membrane ghosts. The expressed proteins were targeted to peroxisomes and peroxisomal ghosts correctly in the presence or absence of Brefeldin A (BFA), a drug known to block the ER-Golgi transit. Furthermore, low temperature did not disturb the targeting of Pex3p-GFP to peroxisomes. We also constructed two chimeric proteins of PMPs containing an ER retention signal "DEKKMP": GFP-ALDRP-DEKKMP and myc- Pex3p-DEKKMP. These proteins were mostly targeted to peroxisomes. No colocalization with an ER maker was found. These results suggest that the classical ER-Golgi pathway does not play a major role in the biogenesis of mammalian PMPs |
| author |
TORO,ANDRÉS ARREDONDO,CRISTIAN CORDOVA,GONZALO ARAYA,CLAUDIA PALACIOS,JOSÉ L VENEGAS,ALEJANDRO MORITA,MASASHI IMANAKA,TSUNEO SANTOS,MANUEL J |
| author_facet |
TORO,ANDRÉS ARREDONDO,CRISTIAN CORDOVA,GONZALO ARAYA,CLAUDIA PALACIOS,JOSÉ L VENEGAS,ALEJANDRO MORITA,MASASHI IMANAKA,TSUNEO SANTOS,MANUEL J |
| author_sort |
TORO,ANDRÉS |
| title |
Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells |
| title_short |
Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells |
| title_full |
Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells |
| title_fullStr |
Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells |
| title_full_unstemmed |
Evaluation of the role of the Endoplasmic Reticulum-Golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant CHO cells |
| title_sort |
evaluation of the role of the endoplasmic reticulum-golgi transit in the biogenesis of peroxisomal membrane proteins in wild type and peroxisome biogenesis mutant cho cells |
| publisher |
Sociedad de Biología de Chile |
| publishDate |
2007 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000200014 |
| work_keys_str_mv |
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