Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations

This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were asse...

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Autores principales:	MOZDARANI,HOSSEIN, MORADI,SHABNAM Z
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2007
Materias:	Cryopreservation vitrification mouse embryo viability chromosome abnormalities
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004
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spelling	oai:scielo:S0716-976020070004000042008-04-17Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durationsMOZDARANI,HOSSEINMORADI,SHABNAM Z Cryopreservation vitrification mouse embryo viability chromosome abnormalities This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration (24 hours, 1 and 2 weeks, 1-6 months) were frozen. After appropriate storage periods embryos were thawed and studied for their viability 4-6 hours after thawing and intact embryos were transferred to fresh medium containing colcemid. After 48 hours, the embryos were fixed and studied for their chromosome abnormalities using Tarkowsky's drying technique. Results indicate that freezing affects the viability and chromosome structure of embryos when compared with the controligroup. Furthermore increasing the storage duration reduces the viability and increases the chromosome aberrations of embryos (such as aneuploidy and polyploidy). This result mµght indicate that the effects of vitrification on the cytoskeleton or other cellular organelle mµght produce chromosomal alterations leading to cell deathinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.40 n.3 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004en10.4067/S0716-97602007000400004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cryopreservation vitrification mouse embryo viability chromosome abnormalities
spellingShingle	Cryopreservation vitrification mouse embryo viability chromosome abnormalities MOZDARANI,HOSSEIN MORADI,SHABNAM Z Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
description	This study was desµgned to investµgate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a controligroup. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of ethyleneiglycol as cryoprotectant. eight-cell mouse embryos were obtained from NMRI mice 3 days after mating. Retrieved embryos were transferred to vitrification solution containing ethyleneiglycol as cryoprotectant, then transferred into a vitrification straw using standard technique, and vitrified in liquid nitrogen. Sixigroups of embryos according to storage duration (24 hours, 1 and 2 weeks, 1-6 months) were frozen. After appropriate storage periods embryos were thawed and studied for their viability 4-6 hours after thawing and intact embryos were transferred to fresh medium containing colcemid. After 48 hours, the embryos were fixed and studied for their chromosome abnormalities using Tarkowsky's drying technique. Results indicate that freezing affects the viability and chromosome structure of embryos when compared with the controligroup. Furthermore increasing the storage duration reduces the viability and increases the chromosome aberrations of embryos (such as aneuploidy and polyploidy). This result mµght indicate that the effects of vitrification on the cytoskeleton or other cellular organelle mµght produce chromosomal alterations leading to cell death
author	MOZDARANI,HOSSEIN MORADI,SHABNAM Z
author_facet	MOZDARANI,HOSSEIN MORADI,SHABNAM Z
author_sort	MOZDARANI,HOSSEIN
title	Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
title_short	Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
title_full	Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
title_fullStr	Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
title_full_unstemmed	Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
title_sort	effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations
publisher	Sociedad de Biología de Chile
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602007000400004
work_keys_str_mv	AT mozdaranihossein effectofvitrificationonviabilityandchromosomeabnormalitiesin8cellmouseembryosatvariousstoragedurations AT moradishabnamz effectofvitrificationonviabilityandchromosomeabnormalitiesin8cellmouseembryosatvariousstoragedurations
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Effect of vitrification on viability and chromosome abnormalities in 8-cell mouse embryos at various storage durations

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