Plant regeneration of natural tetraploid Trifolium Hum pratense L
The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media con...
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Sociedad de Biología de Chile
2008
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oai:scielo:S0716-976020080001000042008-08-21Plant regeneration of natural tetraploid Trifolium Hum pratense LÇÖLGEÇEN,HATICETOKER,M CIHAT red clover (Trifolium pratense L.) Fabaceae organogenesis plant regeneration The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm-3 BAP and 1 mg dm-3 NAA. 94.4% of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm-3 NAA. In vitro organogénesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the fieldinfo:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.1 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100004en10.4067/S0716-97602008000100004 |
institution |
Scielo Chile |
collection |
Scielo Chile |
language |
English |
topic |
red clover (Trifolium pratense L.) Fabaceae organogenesis plant regeneration |
spellingShingle |
red clover (Trifolium pratense L.) Fabaceae organogenesis plant regeneration ÇÖLGEÇEN,HATICE TOKER,M CIHAT Plant regeneration of natural tetraploid Trifolium Hum pratense L |
description |
The regeneration of natural tetraploid T. pratense, originated from Erzurum-Turkey, is reported in this study. This plant has low seed setting and hard seed problems due to polyploidy. Hypocotyl, cotyledon, apical meristems, epicotyl and young primary leaves were inoculated on MS and PC-L2 media containing different concentrations of BAP and NAA as growth regulators. The best shoot formation has been observed on explants initiated from apical meristem placed on PC-L2 medium that includes 2 mg dm-3 BAP and 1 mg dm-3 NAA. 94.4% of the shoots originated from calli were rooted on PC-L2 medium with 1 mg dm-3 NAA. In vitro organogénesis has been accomplished in the natural tetraploid T. pratense regenerated plants successively transferred to the field |
author |
ÇÖLGEÇEN,HATICE TOKER,M CIHAT |
author_facet |
ÇÖLGEÇEN,HATICE TOKER,M CIHAT |
author_sort |
ÇÖLGEÇEN,HATICE |
title |
Plant regeneration of natural tetraploid Trifolium Hum pratense L |
title_short |
Plant regeneration of natural tetraploid Trifolium Hum pratense L |
title_full |
Plant regeneration of natural tetraploid Trifolium Hum pratense L |
title_fullStr |
Plant regeneration of natural tetraploid Trifolium Hum pratense L |
title_full_unstemmed |
Plant regeneration of natural tetraploid Trifolium Hum pratense L |
title_sort |
plant regeneration of natural tetraploid trifolium hum pratense l |
publisher |
Sociedad de Biología de Chile |
publishDate |
2008 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000100004 |
work_keys_str_mv |
AT colgecenhatice plantregenerationofnaturaltetraploidtrifoliumhumpratensel AT tokermcihat plantregenerationofnaturaltetraploidtrifoliumhumpratensel |
_version_ |
1718441427755597824 |