Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line

We have previously characterized a number of small molecule organic compounds that prevent the aggregation of the β-amyloid peptide and its neurotoxicity in hippocampal neuronal cultures. We have now evaluated the effects of such compounds on amyloid precursor protein (APP) accumulation in...

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Autores principales:	LIMA,ANDRÉA C PAULA, ARRIAGADA,CHRISTIAN, TORO,RODRIGO, CÁRDENAS,ANA MARÍA, CAVIEDES,RAÚL, FERREIRA,SERGIO T, CAVIEDES,PABLO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	Alzheimer's disease Down syndrome intracellular amyloid murine trisomy 16 small molecule inhibitors
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200001
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spelling	oai:scielo:S0716-976020080002000012009-03-24Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell lineLIMA,ANDRÉA C PAULAARRIAGADA,CHRISTIANTORO,RODRIGOCÁRDENAS,ANA MARÍACAVIEDES,RAÚLFERREIRA,SERGIO TCAVIEDES,PABLO Alzheimer's disease Down syndrome intracellular amyloid murine trisomy 16 small molecule inhibitors We have previously characterized a number of small molecule organic compounds that prevent the aggregation of the β-amyloid peptide and its neurotoxicity in hippocampal neuronal cultures. We have now evaluated the effects of such compounds on amyloid precursor protein (APP) accumulation in the CTb immortalized cell line derived from the cerebral cortex of a trisomy 16 mouse, an animal model of Down's syndrome. Compared to a non-trisomic cortical cell line (CNh), CTb cells overexpress APP and exhibit slightly elevated resting intracellular Ca2+ levéis ([Ca2+]¡). Here, we show that the compounds 2,4-dinitrophenol, 3-nitrophenol and 4-anisidine decreased intracellular accumulation of APP in CTb cells. Those compounds were non-toxic to the cells, and slightly increased the basal [Ca2+]¡. Results indícate that the compounds tested can be leads for the development of drugs to decrease intracellular vesicular accumulation of APP in trisomic cells.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.2 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200001en10.4067/S0716-97602008000200001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Alzheimer's disease Down syndrome intracellular amyloid murine trisomy 16 small molecule inhibitors
spellingShingle	Alzheimer's disease Down syndrome intracellular amyloid murine trisomy 16 small molecule inhibitors LIMA,ANDRÉA C PAULA ARRIAGADA,CHRISTIAN TORO,RODRIGO CÁRDENAS,ANA MARÍA CAVIEDES,RAÚL FERREIRA,SERGIO T CAVIEDES,PABLO Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
description	We have previously characterized a number of small molecule organic compounds that prevent the aggregation of the β-amyloid peptide and its neurotoxicity in hippocampal neuronal cultures. We have now evaluated the effects of such compounds on amyloid precursor protein (APP) accumulation in the CTb immortalized cell line derived from the cerebral cortex of a trisomy 16 mouse, an animal model of Down's syndrome. Compared to a non-trisomic cortical cell line (CNh), CTb cells overexpress APP and exhibit slightly elevated resting intracellular Ca2+ levéis ([Ca2+]¡). Here, we show that the compounds 2,4-dinitrophenol, 3-nitrophenol and 4-anisidine decreased intracellular accumulation of APP in CTb cells. Those compounds were non-toxic to the cells, and slightly increased the basal [Ca2+]¡. Results indícate that the compounds tested can be leads for the development of drugs to decrease intracellular vesicular accumulation of APP in trisomic cells.
author	LIMA,ANDRÉA C PAULA ARRIAGADA,CHRISTIAN TORO,RODRIGO CÁRDENAS,ANA MARÍA CAVIEDES,RAÚL FERREIRA,SERGIO T CAVIEDES,PABLO
author_facet	LIMA,ANDRÉA C PAULA ARRIAGADA,CHRISTIAN TORO,RODRIGO CÁRDENAS,ANA MARÍA CAVIEDES,RAÚL FERREIRA,SERGIO T CAVIEDES,PABLO
author_sort	LIMA,ANDRÉA C PAULA
title	Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
title_short	Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
title_full	Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
title_fullStr	Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
title_full_unstemmed	Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
title_sort	small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200001
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Small-molecule aggregation inhibitors reduce excess amyloid in a trisomy 16 mouse cortical cell line

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