Occurrence of killer yeast strains in industrial and clinical yeast isolates

The secretion of proteinaceous toxins is a widespread characteristic in environmental and laboratory yeast isolates, a phenomenon called "killer system". The killer phenotype (K+) can be encoded by extrachromosomal genetic elements (EGEs) as double stranded DNA or RNA molecules (dsDNA, dsR...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	BAEZA,MARCELO E, SANHUEZA,MARIO A, CIFUENTES,VÍCTOR H
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	killer system mycotoxin dsRNA
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200007
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602008000200007
record_format	dspace
spelling	oai:scielo:S0716-976020080002000072009-03-24Occurrence of killer yeast strains in industrial and clinical yeast isolatesBAEZA,MARCELO ESANHUEZA,MARIO ACIFUENTES,VÍCTOR H killer system mycotoxin dsRNA The secretion of proteinaceous toxins is a widespread characteristic in environmental and laboratory yeast isolates, a phenomenon called "killer system". The killer phenotype (K+) can be encoded by extrachromosomal genetic elements (EGEs) as double stranded DNA or RNA molecules (dsDNA, dsRNA) or in nuclear genes. The spectrum of action and the activity of killer toxins are influenced by temperature, salinity and pH of media. In the present work we determined the existence of K+ in a collection of S. cerevisiae and P. anómala yeasts isolated from environmental, industrial and clinical sources. The assays were performed in strains belonging to three yeast genera used as sensitive cells and under a wide range of pH and temperatures. Approximately 51 % of isolates tested showed toxicity against at least one sensitive yeast strain under the conditions tested. The K+ P. anómala isolates showed a wide spectrum of action and two of them had toxic activity against strains of the three yeast genera assayed, including C. albicans strains. In all S. cerevisiae K+ isolates an extrachromosomal dsRNA molecule (4.2 Kb) was observed, contrary to P. anómala K+ isolates, which do not possess any EGEs. The K+ phenotype is produced by an exported protein factor and the kinetics of killer activity production was similar in all isolates with high activity in the log phase of growth, decaying in the stationary phase.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.2 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200007en10.4067/S0716-97602008000200007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	killer system mycotoxin dsRNA
spellingShingle	killer system mycotoxin dsRNA BAEZA,MARCELO E SANHUEZA,MARIO A CIFUENTES,VÍCTOR H Occurrence of killer yeast strains in industrial and clinical yeast isolates
description	The secretion of proteinaceous toxins is a widespread characteristic in environmental and laboratory yeast isolates, a phenomenon called "killer system". The killer phenotype (K+) can be encoded by extrachromosomal genetic elements (EGEs) as double stranded DNA or RNA molecules (dsDNA, dsRNA) or in nuclear genes. The spectrum of action and the activity of killer toxins are influenced by temperature, salinity and pH of media. In the present work we determined the existence of K+ in a collection of S. cerevisiae and P. anómala yeasts isolated from environmental, industrial and clinical sources. The assays were performed in strains belonging to three yeast genera used as sensitive cells and under a wide range of pH and temperatures. Approximately 51 % of isolates tested showed toxicity against at least one sensitive yeast strain under the conditions tested. The K+ P. anómala isolates showed a wide spectrum of action and two of them had toxic activity against strains of the three yeast genera assayed, including C. albicans strains. In all S. cerevisiae K+ isolates an extrachromosomal dsRNA molecule (4.2 Kb) was observed, contrary to P. anómala K+ isolates, which do not possess any EGEs. The K+ phenotype is produced by an exported protein factor and the kinetics of killer activity production was similar in all isolates with high activity in the log phase of growth, decaying in the stationary phase.
author	BAEZA,MARCELO E SANHUEZA,MARIO A CIFUENTES,VÍCTOR H
author_facet	BAEZA,MARCELO E SANHUEZA,MARIO A CIFUENTES,VÍCTOR H
author_sort	BAEZA,MARCELO E
title	Occurrence of killer yeast strains in industrial and clinical yeast isolates
title_short	Occurrence of killer yeast strains in industrial and clinical yeast isolates
title_full	Occurrence of killer yeast strains in industrial and clinical yeast isolates
title_fullStr	Occurrence of killer yeast strains in industrial and clinical yeast isolates
title_full_unstemmed	Occurrence of killer yeast strains in industrial and clinical yeast isolates
title_sort	occurrence of killer yeast strains in industrial and clinical yeast isolates
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200007
work_keys_str_mv	AT baezamarceloe occurrenceofkilleryeaststrainsinindustrialandclinicalyeastisolates AT sanhuezamarioa occurrenceofkilleryeaststrainsinindustrialandclinicalyeastisolates AT cifuentesvictorh occurrenceofkilleryeaststrainsinindustrialandclinicalyeastisolates
_version_	1718441432392400896

Occurrence of killer yeast strains in industrial and clinical yeast isolates

Ejemplares similares