Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels

Neurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells dif...

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Autores principales:	CUDDON,PAUL, BOOTMAN,MARTIN D, RICHARDS,GILLIAN R, SMITH,ALISON J, SIMPSON,PETER B, RODERICK,H LLEWELYN
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	Ca2+ PDGF methacholine TRPC6 SOCE human neurosphere-derived cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008
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spelling	oai:scielo:S0716-976020080002000082009-03-24Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channelsCUDDON,PAULBOOTMAN,MARTIN DRICHARDS,GILLIAN RSMITH,ALISON JSIMPSON,PETER BRODERICK,H LLEWELYN Ca2+ PDGF methacholine TRPC6 SOCE human neurosphere-derived cells Neurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells differentiate remain to be fully elucidated. Among the many signáis that impinge on the differentiation of neuronal cells, cytosolic calcium (Ca2+) has an important role. However, little is known about the nature of the Ca2+ signáis involved in fate choice in neuronal precursor cells, or their sources. In this study, we show that activation of either muscarinic or platelet-derived growth factor (PDGF) receptors induces a biphasic increase in cytosolic Ca2+ that consists of reléase from intracellular stores followed by sustained entry across the plasma membrane. For both agonists, the prolonged Ca2+ entry occurred via a store-operated pathway that was pharmacologically indistinguishable from Ca2+ entry initiated by thapsigargin. However, muscarinic receptor-activated Ca2+ entry was inhibited by siRNA-mediated knockdown of TRPC6, whereas Ca2+ entry evoked by PDGF was not. These data provide evidence for agonist-specific activation of molecularly distinct store-operated Ca2+ entry pathways, and raise the possibility of privileged communication between these Ca2+ entry pathways and downstream processes.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.2 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008en10.4067/S0716-97602008000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Ca2+ PDGF methacholine TRPC6 SOCE human neurosphere-derived cells
spellingShingle	Ca2+ PDGF methacholine TRPC6 SOCE human neurosphere-derived cells CUDDON,PAUL BOOTMAN,MARTIN D RICHARDS,GILLIAN R SMITH,ALISON J SIMPSON,PETER B RODERICK,H LLEWELYN Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
description	Neurons are a diverse cell type exhibiting hugely different morphologies and neurotransmitter specifications. Their distinctive phenotypes are established during differentiation from pluripotent precursor cells. The signalling pathways that specify the lineage down which neuronal precursor cells differentiate remain to be fully elucidated. Among the many signáis that impinge on the differentiation of neuronal cells, cytosolic calcium (Ca2+) has an important role. However, little is known about the nature of the Ca2+ signáis involved in fate choice in neuronal precursor cells, or their sources. In this study, we show that activation of either muscarinic or platelet-derived growth factor (PDGF) receptors induces a biphasic increase in cytosolic Ca2+ that consists of reléase from intracellular stores followed by sustained entry across the plasma membrane. For both agonists, the prolonged Ca2+ entry occurred via a store-operated pathway that was pharmacologically indistinguishable from Ca2+ entry initiated by thapsigargin. However, muscarinic receptor-activated Ca2+ entry was inhibited by siRNA-mediated knockdown of TRPC6, whereas Ca2+ entry evoked by PDGF was not. These data provide evidence for agonist-specific activation of molecularly distinct store-operated Ca2+ entry pathways, and raise the possibility of privileged communication between these Ca2+ entry pathways and downstream processes.
author	CUDDON,PAUL BOOTMAN,MARTIN D RICHARDS,GILLIAN R SMITH,ALISON J SIMPSON,PETER B RODERICK,H LLEWELYN
author_facet	CUDDON,PAUL BOOTMAN,MARTIN D RICHARDS,GILLIAN R SMITH,ALISON J SIMPSON,PETER B RODERICK,H LLEWELYN
author_sort	CUDDON,PAUL
title	Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
title_short	Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
title_full	Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
title_fullStr	Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
title_full_unstemmed	Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
title_sort	methacholine and pdgf activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200008
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Methacholine and PDGF activate store-operated calcium entry in neuronal precursor cells via distinct calcium entry channels

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