Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment

Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified link...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	ALATZAS,ANASTASIOS, SREBREVA,LJUBA, FOUNDOULI,ATHINA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	callus culture linker histone plant cell differentiation plant hormone Zea mays
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200010
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602008000200010
record_format	dspace
spelling	oai:scielo:S0716-976020080002000102009-03-24Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatmentALATZAS,ANASTASIOSSREBREVA,LJUBAFOUNDOULI,ATHINA callus culture linker histone plant cell differentiation plant hormone Zea mays Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified linker histone variants by using specific anti-histone Hl antibodies. Each variant's ratio to total Hl in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them was estimated in order to define possible alterations either during plant cell differentiation or during their dedifferentiation. We also evaluated linker histone variants' ratios in the developmental zones of maize roots treated with auxin in order to examine the effects of exogenous applied auxin to linker histone variant distribution. Finally, immunohistochemical detection was used to identify the root tissues containing each variant and correlate them with the physiological status of the plant cells. According to the results presented in this study, linker histone variants' ratios are altered in the developmental zones of maize root, while they are similar to the meristematic zone in samples from callus cultures and to the differentiation zone in samples from roots treated with auxin. We propose that the alterations in linker histone variants' ratios are correlated with plant cell differentiation and dedifferentiation.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.2 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200010en10.4067/S0716-97602008000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	callus culture linker histone plant cell differentiation plant hormone Zea mays
spellingShingle	callus culture linker histone plant cell differentiation plant hormone Zea mays ALATZAS,ANASTASIOS SREBREVA,LJUBA FOUNDOULI,ATHINA Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
description	Although several linker histone variants have been studied in both animal and plant organisms, little is known about their distribution during processes that involve alterations in chromatin function, such as differentiation, dedifferentiation and hormone treatment. In this study, we identified linker histone variants by using specific anti-histone Hl antibodies. Each variant's ratio to total Hl in the three developmental zones of maize (Zea mays L.) root and in callus cultures derived from them was estimated in order to define possible alterations either during plant cell differentiation or during their dedifferentiation. We also evaluated linker histone variants' ratios in the developmental zones of maize roots treated with auxin in order to examine the effects of exogenous applied auxin to linker histone variant distribution. Finally, immunohistochemical detection was used to identify the root tissues containing each variant and correlate them with the physiological status of the plant cells. According to the results presented in this study, linker histone variants' ratios are altered in the developmental zones of maize root, while they are similar to the meristematic zone in samples from callus cultures and to the differentiation zone in samples from roots treated with auxin. We propose that the alterations in linker histone variants' ratios are correlated with plant cell differentiation and dedifferentiation.
author	ALATZAS,ANASTASIOS SREBREVA,LJUBA FOUNDOULI,ATHINA
author_facet	ALATZAS,ANASTASIOS SREBREVA,LJUBA FOUNDOULI,ATHINA
author_sort	ALATZAS,ANASTASIOS
title	Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
title_short	Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
title_full	Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
title_fullStr	Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
title_full_unstemmed	Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
title_sort	distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000200010
work_keys_str_mv	AT alatzasanastasios distributionoflinkerhistonevariantsduringplantcelldifferentiationinthedevelopmentalzonesofthemaizerootdedifferentiationincalluscultureafterauxintreatment AT srebrevaljuba distributionoflinkerhistonevariantsduringplantcelldifferentiationinthedevelopmentalzonesofthemaizerootdedifferentiationincalluscultureafterauxintreatment AT foundouliathina distributionoflinkerhistonevariantsduringplantcelldifferentiationinthedevelopmentalzonesofthemaizerootdedifferentiationincalluscultureafterauxintreatment
_version_	1718441433142132736

Distribution of linker histone variants during plant cell differentiation in the developmental zones of the maize root, dedifferentiation in callus culture after auxin treatment

Ejemplares similares