Hematopoietic potential of mouse placenta with the application of placenta flushing

Several major vascular tissues, such as the aorta-gonad-mesonephros región (AGM), yolk sac, and fetal liver have been confirmed to possess hematopoietic function. Recently, the placenta has been demonstrated as another hematopoietic organ. However, it is not conclusive whether the placenta possesses...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	WEI,BIN, YUAN,SUOMAO, GAO,YINGMAO, BING,LUJUN
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	placenta hematopoiesis HS/PCs mouse
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300003
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602008000300003
record_format	dspace
spelling	oai:scielo:S0716-976020080003000032009-04-23Hematopoietic potential of mouse placenta with the application of placenta flushingWEI,BINYUAN,SUOMAOGAO,YINGMAOBING,LUJUN placenta hematopoiesis HS/PCs mouse Several major vascular tissues, such as the aorta-gonad-mesonephros región (AGM), yolk sac, and fetal liver have been confirmed to possess hematopoietic function. Recently, the placenta has been demonstrated as another hematopoietic organ. However, it is not conclusive whether the placenta possesses hematopoietic ability. Therefore, we undertook a series of experiments to study the hematopoietic functions of placenta. Fetal blood circulation in the placenta is difficult to be eliminated and its interference in the study of placental hematopoiesis is inevitable. With the application of placental flushing, fetal blood contained in the placenta was eliminated. We then made the further study of placental hematopoiesis after the El2.5 placenta was flushed. Our studies showed that placental cells expressing Sca-1, CD117 and CD34 were mainly restricted to the embryonic vessels of E12.5 placenta. The results of fluorescence activated cell sorter (FACs) analysis and colony forming cells (CFC) assay demonstrated that both placenta and placental blood contained hematopoietic stem/progenitor cells (HS/PCs), including CFU-GMs, CFU-GEMMs, BFU-Es, and HPP-CFCs. The frequency of HS/PCs in the placenta was 2-3 times that of placental blood. Therefore, it is necessary to clear placental blood out of the placenta in the studies of the hematopoietic potential of placenta. The placenta still possessed the hematopoietic potential after the fetal blood is flushed out. These observations provide further evidences that the placenta is a hematopoietic organ, as has been proposed for other embryonic hematopoietic sites.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.3 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300003en10.4067/S0716-97602008000300003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	placenta hematopoiesis HS/PCs mouse
spellingShingle	placenta hematopoiesis HS/PCs mouse WEI,BIN YUAN,SUOMAO GAO,YINGMAO BING,LUJUN Hematopoietic potential of mouse placenta with the application of placenta flushing
description	Several major vascular tissues, such as the aorta-gonad-mesonephros región (AGM), yolk sac, and fetal liver have been confirmed to possess hematopoietic function. Recently, the placenta has been demonstrated as another hematopoietic organ. However, it is not conclusive whether the placenta possesses hematopoietic ability. Therefore, we undertook a series of experiments to study the hematopoietic functions of placenta. Fetal blood circulation in the placenta is difficult to be eliminated and its interference in the study of placental hematopoiesis is inevitable. With the application of placental flushing, fetal blood contained in the placenta was eliminated. We then made the further study of placental hematopoiesis after the El2.5 placenta was flushed. Our studies showed that placental cells expressing Sca-1, CD117 and CD34 were mainly restricted to the embryonic vessels of E12.5 placenta. The results of fluorescence activated cell sorter (FACs) analysis and colony forming cells (CFC) assay demonstrated that both placenta and placental blood contained hematopoietic stem/progenitor cells (HS/PCs), including CFU-GMs, CFU-GEMMs, BFU-Es, and HPP-CFCs. The frequency of HS/PCs in the placenta was 2-3 times that of placental blood. Therefore, it is necessary to clear placental blood out of the placenta in the studies of the hematopoietic potential of placenta. The placenta still possessed the hematopoietic potential after the fetal blood is flushed out. These observations provide further evidences that the placenta is a hematopoietic organ, as has been proposed for other embryonic hematopoietic sites.
author	WEI,BIN YUAN,SUOMAO GAO,YINGMAO BING,LUJUN
author_facet	WEI,BIN YUAN,SUOMAO GAO,YINGMAO BING,LUJUN
author_sort	WEI,BIN
title	Hematopoietic potential of mouse placenta with the application of placenta flushing
title_short	Hematopoietic potential of mouse placenta with the application of placenta flushing
title_full	Hematopoietic potential of mouse placenta with the application of placenta flushing
title_fullStr	Hematopoietic potential of mouse placenta with the application of placenta flushing
title_full_unstemmed	Hematopoietic potential of mouse placenta with the application of placenta flushing
title_sort	hematopoietic potential of mouse placenta with the application of placenta flushing
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300003
work_keys_str_mv	AT weibin hematopoieticpotentialofmouseplacentawiththeapplicationofplacentaflushing AT yuansuomao hematopoieticpotentialofmouseplacentawiththeapplicationofplacentaflushing AT gaoyingmao hematopoieticpotentialofmouseplacentawiththeapplicationofplacentaflushing AT binglujun hematopoieticpotentialofmouseplacentawiththeapplicationofplacentaflushing
_version_	1718441434594410496

Hematopoietic potential of mouse placenta with the application of placenta flushing

Ejemplares similares