$Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates$
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Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates

The in vitro antibacterial activity of crude methanolic extract of the seeds of Garcinia kola was investigated. The extracts exhibited antibacterial activities with zones of inhibition ranging from 10 mm to 25 mm. The minimum inhibitory concentration of the diethyl ether fraction was between 0.313 a...

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Autores principales:	AKINPELU,DA, ADEGBOYE,MF, ADELOYE,OA, OKOH,AI
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	Garcinia kola antimicrobial activity MIC killing rate protein leakage
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300005
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spelling	oai:scielo:S0716-976020080003000052009-04-23Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolatesAKINPELU,DAADEGBOYE,MFADELOYE,OAOKOH,AI Garcinia kola antimicrobial activity MIC killing rate protein leakage The in vitro antibacterial activity of crude methanolic extract of the seeds of Garcinia kola was investigated. The extracts exhibited antibacterial activities with zones of inhibition ranging from 10 mm to 25 mm. The minimum inhibitory concentration of the diethyl ether fraction was between 0.313 and 5.0 mg/ml, while that of butanol fraction varied from 0.157 to 5.0 mg/ml. The butanol fraction killed about 77% of Bacillus anthracis and 79% of Escherichia coli cells within 120 min at a concentration of 5.0 mg/ml. Protein leakage from the B. anthracis and E. coli cells when exposed to the butanol and diethyl ether fractions was observed. We conclude that Garcinia kola seed extract has a broad spectrum antibacterial activity, with the butanol and diethyl ether fractions being bactericidal as exemplified by the killing rate and protein leakage regimes, which suggest cell membrane disruption as a mechanism of action of the extract.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.3 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300005en10.4067/S0716-97602008000300005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Garcinia kola antimicrobial activity MIC killing rate protein leakage
spellingShingle	Garcinia kola antimicrobial activity MIC killing rate protein leakage AKINPELU,DA ADEGBOYE,MF ADELOYE,OA OKOH,AI Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates
description	The in vitro antibacterial activity of crude methanolic extract of the seeds of Garcinia kola was investigated. The extracts exhibited antibacterial activities with zones of inhibition ranging from 10 mm to 25 mm. The minimum inhibitory concentration of the diethyl ether fraction was between 0.313 and 5.0 mg/ml, while that of butanol fraction varied from 0.157 to 5.0 mg/ml. The butanol fraction killed about 77% of Bacillus anthracis and 79% of Escherichia coli cells within 120 min at a concentration of 5.0 mg/ml. Protein leakage from the B. anthracis and E. coli cells when exposed to the butanol and diethyl ether fractions was observed. We conclude that Garcinia kola seed extract has a broad spectrum antibacterial activity, with the butanol and diethyl ether fractions being bactericidal as exemplified by the killing rate and protein leakage regimes, which suggest cell membrane disruption as a mechanism of action of the extract.
author	AKINPELU,DA ADEGBOYE,MF ADELOYE,OA OKOH,AI
author_facet	AKINPELU,DA ADEGBOYE,MF ADELOYE,OA OKOH,AI
author_sort	AKINPELU,DA
title	Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates
title_short	Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates
title_full	Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates
title_fullStr	Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates
title_full_unstemmed	Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates
title_sort	biocidal activity of partially purified fractions from methanolic extract of garcinia kola (heckel) seeds on bacterial isolates
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300005
work_keys_str_mv	AT akinpeluda biocidalactivityofpartiallypurifiedfractionsfrommethanolicextractofgarciniakolaheckelseedsonbacterialisolates AT adegboyemf biocidalactivityofpartiallypurifiedfractionsfrommethanolicextractofgarciniakolaheckelseedsonbacterialisolates AT adeloyeoa biocidalactivityofpartiallypurifiedfractionsfrommethanolicextractofgarciniakolaheckelseedsonbacterialisolates AT okohai biocidalactivityofpartiallypurifiedfractionsfrommethanolicextractofgarciniakolaheckelseedsonbacterialisolates
_version_	1718441435188953088

Biocidal activity of partially purified fractions from methanolic extract of Garcinia kola (Heckel) seeds on bacterial isolates

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