Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression

Carotenoids are synthesized in prokaryotic and eukaryotic organisms. In plants and algae, these lipophilic molecules possess antioxidant properties acting as reactive oxygen species scavengers and exert functional roles in hormone synthesis, photosynthesis, photomorphogenesis and in photoprotection....

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	STANGE,CLAUDIA, FUENTES,PAULINA, HANDFORD,MICHAEL, PIZARRO,LORENA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	carotenoid biosynthesis carrot gene expression light regulation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300006
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602008000300006
record_format	dspace
spelling	oai:scielo:S0716-976020080003000062009-04-23Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expressionSTANGE,CLAUDIAFUENTES,PAULINAHANDFORD,MICHAELPIZARRO,LORENA carotenoid biosynthesis carrot gene expression light regulation Carotenoids are synthesized in prokaryotic and eukaryotic organisms. In plants and algae, these lipophilic molecules possess antioxidant properties acting as reactive oxygen species scavengers and exert functional roles in hormone synthesis, photosynthesis, photomorphogenesis and in photoprotection. During the past decade almost all carotenogenic genes have been identified as a result of molecular, genetic and biochemical approaches utilizing Arabidopsis thaliana as the model system. Studies carried out in leaves and fruits of A. thaliana and tomato determined that light regulates carotenoid biosynthesis preferentially through the modulation of carotenogenic gene transcription. In this work we showed for the first time that light induces accumulation of psy 1, pds and zds2 transcripts in leaves of Daucus carota (carrot), a novel plant model. In addition, modified roots of carrots exposed to light accumulate zdsl, whereas the pds gene is highly repressed, suggesting that some carotenogenic genes, which are expressed in roots, are regulated by light. Additionally, light negatively regulates the development of the modified carrot root in a reversible manner. Therefore, this suggests that light affects normal growth and carotenogenic gene expression in the modified root of carrot plants. The molecular insight gained into the light-regulated expression of carotenoid genes in this and other model systems will facilitate our understanding of the regulation of carotenoid biosynthesis to improve the prospects for the metabolic engineering of carotenoid production in plants.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.3 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300006en10.4067/S0716-97602008000300006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	carotenoid biosynthesis carrot gene expression light regulation
spellingShingle	carotenoid biosynthesis carrot gene expression light regulation STANGE,CLAUDIA FUENTES,PAULINA HANDFORD,MICHAEL PIZARRO,LORENA Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
description	Carotenoids are synthesized in prokaryotic and eukaryotic organisms. In plants and algae, these lipophilic molecules possess antioxidant properties acting as reactive oxygen species scavengers and exert functional roles in hormone synthesis, photosynthesis, photomorphogenesis and in photoprotection. During the past decade almost all carotenogenic genes have been identified as a result of molecular, genetic and biochemical approaches utilizing Arabidopsis thaliana as the model system. Studies carried out in leaves and fruits of A. thaliana and tomato determined that light regulates carotenoid biosynthesis preferentially through the modulation of carotenogenic gene transcription. In this work we showed for the first time that light induces accumulation of psy 1, pds and zds2 transcripts in leaves of Daucus carota (carrot), a novel plant model. In addition, modified roots of carrots exposed to light accumulate zdsl, whereas the pds gene is highly repressed, suggesting that some carotenogenic genes, which are expressed in roots, are regulated by light. Additionally, light negatively regulates the development of the modified carrot root in a reversible manner. Therefore, this suggests that light affects normal growth and carotenogenic gene expression in the modified root of carrot plants. The molecular insight gained into the light-regulated expression of carotenoid genes in this and other model systems will facilitate our understanding of the regulation of carotenoid biosynthesis to improve the prospects for the metabolic engineering of carotenoid production in plants.
author	STANGE,CLAUDIA FUENTES,PAULINA HANDFORD,MICHAEL PIZARRO,LORENA
author_facet	STANGE,CLAUDIA FUENTES,PAULINA HANDFORD,MICHAEL PIZARRO,LORENA
author_sort	STANGE,CLAUDIA
title	Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
title_short	Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
title_full	Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
title_fullStr	Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
title_full_unstemmed	Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
title_sort	daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300006
work_keys_str_mv	AT stangeclaudia daucuscarotaasanovelmodeltoevaluatetheeffectoflightoncarotenogenicgeneexpression AT fuentespaulina daucuscarotaasanovelmodeltoevaluatetheeffectoflightoncarotenogenicgeneexpression AT handfordmichael daucuscarotaasanovelmodeltoevaluatetheeffectoflightoncarotenogenicgeneexpression AT pizarrolorena daucuscarotaasanovelmodeltoevaluatetheeffectoflightoncarotenogenicgeneexpression
_version_	1718441435430125568

Daucus carota as a novel model to evaluate the effect of light on carotenogenic gene expression

Ejemplares similares