DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis

Diffuse type gastric carcinoma is the most aggressive type of gastric cáncer. This type of tumor is not preceded by precancerous changes and is associated with early-onset and hereditary syndromes. To test the hypothesis that DNA methylation profile would be useful for molecular classification of th...

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Autores principales:	BERNAL,CAROLINA, VARGAS,MACARENA, OSSANDÓN,FRANCISCO, SANTIBÁÑEZ,EUDOCIA, URRUTIA,JULIO, LUENGO,VÍCTOR, ZAVALA,LUIS F, BACKHOUSE,CLAUDIA, PALMA,MARIANA, ARGANDOÑA,JORGE, AGUAYO,FRANCISCO, CORVALÁN,ALEJANDRO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	gastric cáncer diffuse type early-onset BRCA-1
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300007
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spelling	oai:scielo:S0716-976020080003000072009-04-23DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesisBERNAL,CAROLINAVARGAS,MACARENAOSSANDÓN,FRANCISCOSANTIBÁÑEZ,EUDOCIAURRUTIA,JULIOLUENGO,VÍCTORZAVALA,LUIS FBACKHOUSE,CLAUDIAPALMA,MARIANAARGANDOÑA,JORGEAGUAYO,FRANCISCOCORVALÁN,ALEJANDRO gastric cáncer diffuse type early-onset BRCA-1 Diffuse type gastric carcinoma is the most aggressive type of gastric cáncer. This type of tumor is not preceded by precancerous changes and is associated with early-onset and hereditary syndromes. To test the hypothesis that DNA methylation profile would be useful for molecular classification of the diffuse type gastric carcinoma, DNA methylation patterns of the CpG Island of 17 genes were studied in 104 cases and 47 normal adjacent gastric mucosa by Methylation-specific PCR, Immunohistochemistry and Hierarchical clustering analysis. The most frequent methylated genes were FHIT, E-cadherin, BRCA1 and APC (>50%), followed by pl4, pl6, pl5, p73, MGMT and SEMA3B (20-49%). Hierarchical clustering analysis reveáis four groups with different clinical features. The first was characterized by hypermethylation of BRCA1 and younger age (<45 years oíd), and the second by hypermethylation of pl4 and pl6 genes, male predominance and Epstein-Barr virus infection. The third group was characterized by hypermethylation of FHIT and antrum located tumors and the fourth was not associated with any clinical variables. In normal adjacent mucosa only the p73 gene was significantly less methylated in comparison to tumor mucosa. DNA methylation identified subgroups of diffuse type gastric cáncer. Hypermethylation of BRCA1 associated with young age suggests a role in early-onset gastric carcinoma.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.3 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300007en10.4067/S0716-97602008000300007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	gastric cáncer diffuse type early-onset BRCA-1
spellingShingle	gastric cáncer diffuse type early-onset BRCA-1 BERNAL,CAROLINA VARGAS,MACARENA OSSANDÓN,FRANCISCO SANTIBÁÑEZ,EUDOCIA URRUTIA,JULIO LUENGO,VÍCTOR ZAVALA,LUIS F BACKHOUSE,CLAUDIA PALMA,MARIANA ARGANDOÑA,JORGE AGUAYO,FRANCISCO CORVALÁN,ALEJANDRO DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis
description	Diffuse type gastric carcinoma is the most aggressive type of gastric cáncer. This type of tumor is not preceded by precancerous changes and is associated with early-onset and hereditary syndromes. To test the hypothesis that DNA methylation profile would be useful for molecular classification of the diffuse type gastric carcinoma, DNA methylation patterns of the CpG Island of 17 genes were studied in 104 cases and 47 normal adjacent gastric mucosa by Methylation-specific PCR, Immunohistochemistry and Hierarchical clustering analysis. The most frequent methylated genes were FHIT, E-cadherin, BRCA1 and APC (>50%), followed by pl4, pl6, pl5, p73, MGMT and SEMA3B (20-49%). Hierarchical clustering analysis reveáis four groups with different clinical features. The first was characterized by hypermethylation of BRCA1 and younger age (<45 years oíd), and the second by hypermethylation of pl4 and pl6 genes, male predominance and Epstein-Barr virus infection. The third group was characterized by hypermethylation of FHIT and antrum located tumors and the fourth was not associated with any clinical variables. In normal adjacent mucosa only the p73 gene was significantly less methylated in comparison to tumor mucosa. DNA methylation identified subgroups of diffuse type gastric cáncer. Hypermethylation of BRCA1 associated with young age suggests a role in early-onset gastric carcinoma.
author	BERNAL,CAROLINA VARGAS,MACARENA OSSANDÓN,FRANCISCO SANTIBÁÑEZ,EUDOCIA URRUTIA,JULIO LUENGO,VÍCTOR ZAVALA,LUIS F BACKHOUSE,CLAUDIA PALMA,MARIANA ARGANDOÑA,JORGE AGUAYO,FRANCISCO CORVALÁN,ALEJANDRO
author_facet	BERNAL,CAROLINA VARGAS,MACARENA OSSANDÓN,FRANCISCO SANTIBÁÑEZ,EUDOCIA URRUTIA,JULIO LUENGO,VÍCTOR ZAVALA,LUIS F BACKHOUSE,CLAUDIA PALMA,MARIANA ARGANDOÑA,JORGE AGUAYO,FRANCISCO CORVALÁN,ALEJANDRO
author_sort	BERNAL,CAROLINA
title	DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis
title_short	DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis
title_full	DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis
title_fullStr	DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis
title_full_unstemmed	DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis
title_sort	dna methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the brca1 promoter region in early-onset gastric carcinogenesis
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300007
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DNA methylation profile in diffuse type gastric cancer: evidence for hypermethylation of the BRCA1 promoter region in early-onset gastric carcinogenesis

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