Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli

Corynebacterium glutamicum is widely used in the industrial production of amino acids. We have found that this bacterium grows exponentially on a mineral médium supplemented with gluconate. Gluconate permease and Gluconokinase are expressed in an inducible form and, 6-phosphogluconate dehydrogenase,...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	PORCO,ANTONIETTA, GAMERO,ELIDA E, MYLONÁS,ELENA, ISTÚRIZ,TOMÁS
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2008
Materias:	Corynebacterium glutamicum gluconate metabolism gnt
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300011
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602008000300011
record_format	dspace
spelling	oai:scielo:S0716-976020080003000112009-04-23Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coliPORCO,ANTONIETTAGAMERO,ELIDA EMYLONÁS,ELENAISTÚRIZ,TOMÁS Corynebacterium glutamicum gluconate metabolism gnt Corynebacterium glutamicum is widely used in the industrial production of amino acids. We have found that this bacterium grows exponentially on a mineral médium supplemented with gluconate. Gluconate permease and Gluconokinase are expressed in an inducible form and, 6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3-fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions. Interestingly, these activities are lower than those detected in the strain Escherichia coli Ml-8, cultivated under similar conditions. Additionally, here we also confirmed that this bacterium lacks 6-phosphogluconate dehydratase activity. Thus, gluconate must be metabolized through the pentose phosphate pathway. Genes encoding gluconate transport and its phosphorylation were cloned from C. glutamicum, and expressed in suitable E. coli mutants. Sequence analysis revealed that the amino acid sequences obtained from these genes, denoted as gntP and gntK, were similar to those found in other bacteria. Analysis of both genes by RT-PCR suggested constitutive expression, in disagreement with the inducible character of their corresponding activities. The results suggest that gluconate might be a suitable source of reduction potential for improving the efficiency in cultures engaged in amino acids production. This is the first time that gluconate specific enzymatic activities are reported in C. glutamicum.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.41 n.3 20082008-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300011en10.4067/S0716-97602008000300011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Corynebacterium glutamicum gluconate metabolism gnt
spellingShingle	Corynebacterium glutamicum gluconate metabolism gnt PORCO,ANTONIETTA GAMERO,ELIDA E MYLONÁS,ELENA ISTÚRIZ,TOMÁS Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli
description	Corynebacterium glutamicum is widely used in the industrial production of amino acids. We have found that this bacterium grows exponentially on a mineral médium supplemented with gluconate. Gluconate permease and Gluconokinase are expressed in an inducible form and, 6-phosphogluconate dehydrogenase, although constituvely expressed, shows a 3-fold higher specific level in gluconate grown cells than those grown in fructose under similar conditions. Interestingly, these activities are lower than those detected in the strain Escherichia coli Ml-8, cultivated under similar conditions. Additionally, here we also confirmed that this bacterium lacks 6-phosphogluconate dehydratase activity. Thus, gluconate must be metabolized through the pentose phosphate pathway. Genes encoding gluconate transport and its phosphorylation were cloned from C. glutamicum, and expressed in suitable E. coli mutants. Sequence analysis revealed that the amino acid sequences obtained from these genes, denoted as gntP and gntK, were similar to those found in other bacteria. Analysis of both genes by RT-PCR suggested constitutive expression, in disagreement with the inducible character of their corresponding activities. The results suggest that gluconate might be a suitable source of reduction potential for improving the efficiency in cultures engaged in amino acids production. This is the first time that gluconate specific enzymatic activities are reported in C. glutamicum.
author	PORCO,ANTONIETTA GAMERO,ELIDA E MYLONÁS,ELENA ISTÚRIZ,TOMÁS
author_facet	PORCO,ANTONIETTA GAMERO,ELIDA E MYLONÁS,ELENA ISTÚRIZ,TOMÁS
author_sort	PORCO,ANTONIETTA
title	Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli
title_short	Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli
title_full	Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli
title_fullStr	Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli
title_full_unstemmed	Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli
title_sort	gluconate as suitable potential reduction supplier in corynebacterium glutamicum: cloning and expression of gntp and gntk in escherichia coli
publisher	Sociedad de Biología de Chile
publishDate	2008
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602008000300011
work_keys_str_mv	AT porcoantonietta gluconateassuitablepotentialreductionsupplierincorynebacteriumglutamicumcloningandexpressionofgntpandgntkinescherichiacoli AT gameroelidae gluconateassuitablepotentialreductionsupplierincorynebacteriumglutamicumcloningandexpressionofgntpandgntkinescherichiacoli AT mylonaselena gluconateassuitablepotentialreductionsupplierincorynebacteriumglutamicumcloningandexpressionofgntpandgntkinescherichiacoli AT isturiztomas gluconateassuitablepotentialreductionsupplierincorynebacteriumglutamicumcloningandexpressionofgntpandgntkinescherichiacoli
_version_	1718441436728262656

Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli

Ejemplares similares