Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires

Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density an...

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Autores principales:	SCHREIER,STEFAN, TRIAMPO,WANNAPONG, DOUNGCHAWEE,GALAYANEE, TRIAMPO,DARAPOND, CHADSUTHI,SUDARAT
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	leptospirosis leptospira enumeration spectroscopy indirect counting
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001
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spelling	oai:scielo:S0716-976020090001000012009-06-11Leptospirosis research: fast, easy and reliable enumeration of mobile leptospiresSCHREIER,STEFANTRIAMPO,WANNAPONGDOUNGCHAWEE,GALAYANEETRIAMPO,DARAPONDCHADSUTHI,SUDARAT leptospirosis leptospira enumeration spectroscopy indirect counting Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique for leptospirosis research.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001en10.4067/S0716-97602009000100001
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	leptospirosis leptospira enumeration spectroscopy indirect counting
spellingShingle	leptospirosis leptospira enumeration spectroscopy indirect counting SCHREIER,STEFAN TRIAMPO,WANNAPONG DOUNGCHAWEE,GALAYANEE TRIAMPO,DARAPOND CHADSUTHI,SUDARAT Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
description	Leptospirosis caused by Leptospira interrogans is the most widespread zoonosis and a major public health problem worldwide. Based on light-scattering and absorption, quantification of leptospires using UV-VIS spectroscopy was used as an indirect counting technique by measuring the optical density and comparing this to automated direct counting using a counting chamber in combination with imaging and analyzing software. Two serovars, Bangkok and Copenhagenii, from log-phase growth were used for the establishment of standard curves. They were found to be linear and slightly different in gradient for each serovar. The ease, rapidity, and reliability of these two adapted and optimized counting techniques may provide a useful alternative enumeration technique for leptospirosis research.
author	SCHREIER,STEFAN TRIAMPO,WANNAPONG DOUNGCHAWEE,GALAYANEE TRIAMPO,DARAPOND CHADSUTHI,SUDARAT
author_facet	SCHREIER,STEFAN TRIAMPO,WANNAPONG DOUNGCHAWEE,GALAYANEE TRIAMPO,DARAPOND CHADSUTHI,SUDARAT
author_sort	SCHREIER,STEFAN
title	Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
title_short	Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
title_full	Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
title_fullStr	Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
title_full_unstemmed	Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
title_sort	leptospirosis research: fast, easy and reliable enumeration of mobile leptospires
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100001
work_keys_str_mv	AT schreierstefan leptospirosisresearchfasteasyandreliableenumerationofmobileleptospires AT triampowannapong leptospirosisresearchfasteasyandreliableenumerationofmobileleptospires AT doungchaweegalayanee leptospirosisresearchfasteasyandreliableenumerationofmobileleptospires AT triampodarapond leptospirosisresearchfasteasyandreliableenumerationofmobileleptospires AT chadsuthisudarat leptospirosisresearchfasteasyandreliableenumerationofmobileleptospires
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Leptospirosis research: fast, easy and reliable enumeration of mobile leptospires

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