Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats

Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats

The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. However, despite the beneficial effects on health, concern has been raised that this isoflavone also acts as an endocrine-...

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Autores principales: AJDŽANOVI,VLADIMIR Z, ŠOŠI-JURJEVI,BRANKA T, FILIPÓVI,BRANKO R, TRIFUNOVI,SVETLANA L, BRKIC,DEJAN D, SEKULI,MILKA I, MILOSEVI,VERICA LJ
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2009
Materias:
ACTH cells
corticosterone
genistein
middle-age
orchidectomy
rats
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100002
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spelling oai:scielo:S0716-976020090001000022009-06-11Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male ratsAJDŽANOVI,VLADIMIR ZŠOŠI-JURJEVI,BRANKA TFILIPÓVI,BRANKO RTRIFUNOVI,SVETLANA LBRKIC,DEJAN DSEKULI,MILKA IMILOSEVI,VERICA LJ ACTH cells corticosterone genistein middle-age orchidectomy rats The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. However, despite the beneficial effects on health, concern has been raised that this isoflavone also acts as an endocrine-disrupting chemical. The aim of this study was to examine the effects of genistein on immunohistomorphometric features of pituitary adrenocorticotropic cells (ACTH) and blood concentrations of ACTH and corticosterone in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. ACTH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Circulating concentrations of ACTH and corticosterone were measured by immunoassay. Orchidectomy reduced (p<0.05) the cell volume and the relative volume of ACTH cells in comparison to SO rats. Genistein treatment further decreased (p<0.05) these morphometric parameters and reduced (p<0.05) circulating ACTH and corticosterone concentrations by more than 20% in comparison to both Orx and SO rats. In conclusión, genistein modulated the immunohistomorphometric features of ACTH cells and decreased blood ACTH and corticosterone levels, which supports evidence that this isoflavone affects the hypothalamic-pituitary-adrenal axis and suppresses glucocorticoid hormone secretion.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100002en10.4067/S0716-97602009000100002
institution Scielo Chile
collection Scielo Chile
language English
topic ACTH cells
corticosterone
genistein
middle-age
orchidectomy
rats
spellingShingle ACTH cells
corticosterone
genistein
middle-age
orchidectomy
rats
AJDŽANOVI,VLADIMIR Z
ŠOŠI-JURJEVI,BRANKA T
FILIPÓVI,BRANKO R
TRIFUNOVI,SVETLANA L
BRKIC,DEJAN D
SEKULI,MILKA I
MILOSEVI,VERICA LJ
Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
description The soybean phytoestrogen, genistein, is increasingly consumed as an alternative therapeutic for age-related diseases, namely cardiovascular conditions, cancer and osteoporosis. However, despite the beneficial effects on health, concern has been raised that this isoflavone also acts as an endocrine-disrupting chemical. The aim of this study was to examine the effects of genistein on immunohistomorphometric features of pituitary adrenocorticotropic cells (ACTH) and blood concentrations of ACTH and corticosterone in orchidectomized middle-aged male rats. Sixteen-month-old Wistar rats were divided into sham-operated (SO), orchidectomized (Orx) and genistein-treated orchidectomized (Orx+G) groups. Genistein (30mg/kg/day) was administered subcutaneously for three weeks, while the control groups received the vehicle alone. ACTH cells were identified by the peroxidase-antiperoxidase (PAP) immunohistochemical procedure. Circulating concentrations of ACTH and corticosterone were measured by immunoassay. Orchidectomy reduced (p<0.05) the cell volume and the relative volume of ACTH cells in comparison to SO rats. Genistein treatment further decreased (p<0.05) these morphometric parameters and reduced (p<0.05) circulating ACTH and corticosterone concentrations by more than 20% in comparison to both Orx and SO rats. In conclusión, genistein modulated the immunohistomorphometric features of ACTH cells and decreased blood ACTH and corticosterone levels, which supports evidence that this isoflavone affects the hypothalamic-pituitary-adrenal axis and suppresses glucocorticoid hormone secretion.
author AJDŽANOVI,VLADIMIR Z
ŠOŠI-JURJEVI,BRANKA T
FILIPÓVI,BRANKO R
TRIFUNOVI,SVETLANA L
BRKIC,DEJAN D
SEKULI,MILKA I
MILOSEVI,VERICA LJ
author_facet AJDŽANOVI,VLADIMIR Z
ŠOŠI-JURJEVI,BRANKA T
FILIPÓVI,BRANKO R
TRIFUNOVI,SVETLANA L
BRKIC,DEJAN D
SEKULI,MILKA I
MILOSEVI,VERICA LJ
author_sort AJDŽANOVI,VLADIMIR Z
title Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
title_short Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
title_full Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
title_fullStr Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
title_full_unstemmed Genistein affects the morphology of pituitary ACTH cells and decreases circulating levels of ACTH and corticosterone in middle-aged male rats
title_sort genistein affects the morphology of pituitary acth cells and decreases circulating levels of acth and corticosterone in middle-aged male rats
publisher Sociedad de Biología de Chile
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100002
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