Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display

In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due...

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Autores principales:	SIQUIEROLI,ANA CAROLINA S, VIEIRA,CARLOS U, CARVALHO-ZILSE,GISLENE A, GOULART,LUIZ R, KERR,WARWICK E, BONETTI,ANA M
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	determination of castes intercastes Melipona sp
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100011
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spelling	oai:scielo:S0716-976020090001000112009-06-11Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential DisplaySIQUIEROLI,ANA CAROLINA SVIEIRA,CARLOS UCARVALHO-ZILSE,GISLENE AGOULART,LUIZ RKERR,WARWICK EBONETTI,ANA M determination of castes intercastes Melipona sp In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA pro files of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100011en10.4067/S0716-97602009000100011
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	determination of castes intercastes Melipona sp
spellingShingle	determination of castes intercastes Melipona sp SIQUIEROLI,ANA CAROLINA S VIEIRA,CARLOS U CARVALHO-ZILSE,GISLENE A GOULART,LUIZ R KERR,WARWICK E BONETTI,ANA M Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display
description	In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA pro files of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers.
author	SIQUIEROLI,ANA CAROLINA S VIEIRA,CARLOS U CARVALHO-ZILSE,GISLENE A GOULART,LUIZ R KERR,WARWICK E BONETTI,ANA M
author_facet	SIQUIEROLI,ANA CAROLINA S VIEIRA,CARLOS U CARVALHO-ZILSE,GISLENE A GOULART,LUIZ R KERR,WARWICK E BONETTI,ANA M
author_sort	SIQUIEROLI,ANA CAROLINA S
title	Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display
title_short	Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display
title_full	Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display
title_fullStr	Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display
title_full_unstemmed	Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display
title_sort	analysis of the intercaste transcriptional profile of melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mrna differential display
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100011
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Analysis of the Intercaste Transcriptional Profile of Melipona scutellaris Latreille, 1811 (Hymenoptera, Apidae, Meliponini) by mRNA Differential Display

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