Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla

Endothelial cells are directly involved in many functions of the cardiovascular system by regulating blood flow and blood pressure through Ca2+ dependent exocitosis of vasoactive compounds. Using the Ca2+ indicator Fluo-3 and the patch-clamp technique, we show that bovine adrenal medulla capillary e...

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Autores principales: VINET,RAÚL, CORTÉS,MAGDALENA, LUXORO,MARIO, DELPIANO,MARCO A
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2009
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100012
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spelling oai:scielo:S0716-976020090001000122009-06-11Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medullaVINET,RAÚLCORTÉS,MAGDALENALUXORO,MARIODELPIANO,MARCO A capillary endothelial cells intracellular Ca2+ nicotinic receptor voltage-activated Ca2+ channels Endothelial cells are directly involved in many functions of the cardiovascular system by regulating blood flow and blood pressure through Ca2+ dependent exocitosis of vasoactive compounds. Using the Ca2+ indicator Fluo-3 and the patch-clamp technique, we show that bovine adrenal medulla capillary endothelial cells (B AMCECs) respond to acetylcholine (ACh) with a cytosolic Ca2+ increase and depolarization of the membrane potential (20.3±0.9 mV; n=23). The increase in cytosolic Ca2+ induced by 10µM ACh was mimicked by the same concentration of nicotine but not by muscarine and was blocked by 100 µM of hexamethonium. On the other hand, the increase in cytosolic Ca2+ could be depressed by nifedipine (0.01 -100 µM) or withdrawal of extracellular Ca2+. Taken together, these results give evidence for functional nicotinic receptors (nAChRs) in capillary endothelial cells of the adrenal medulla. It suggests that nAChRs in B AMCECs may be involved in the regulation of the adrenal gland's microcirculation by depolarizing the membrane potential, leading to the opening of voltage-activated Ca2+ channels, influx of external Ca2+ and liberation of vasoactive compounds.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.1 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100012en10.4067/S0716-97602009000100012
institution Scielo Chile
collection Scielo Chile
language English
topic capillary
endothelial cells
intracellular Ca2+
nicotinic receptor
voltage-activated Ca2+ channels
spellingShingle capillary
endothelial cells
intracellular Ca2+
nicotinic receptor
voltage-activated Ca2+ channels
VINET,RAÚL
CORTÉS,MAGDALENA
LUXORO,MARIO
DELPIANO,MARCO A
Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
description Endothelial cells are directly involved in many functions of the cardiovascular system by regulating blood flow and blood pressure through Ca2+ dependent exocitosis of vasoactive compounds. Using the Ca2+ indicator Fluo-3 and the patch-clamp technique, we show that bovine adrenal medulla capillary endothelial cells (B AMCECs) respond to acetylcholine (ACh) with a cytosolic Ca2+ increase and depolarization of the membrane potential (20.3±0.9 mV; n=23). The increase in cytosolic Ca2+ induced by 10µM ACh was mimicked by the same concentration of nicotine but not by muscarine and was blocked by 100 µM of hexamethonium. On the other hand, the increase in cytosolic Ca2+ could be depressed by nifedipine (0.01 -100 µM) or withdrawal of extracellular Ca2+. Taken together, these results give evidence for functional nicotinic receptors (nAChRs) in capillary endothelial cells of the adrenal medulla. It suggests that nAChRs in B AMCECs may be involved in the regulation of the adrenal gland's microcirculation by depolarizing the membrane potential, leading to the opening of voltage-activated Ca2+ channels, influx of external Ca2+ and liberation of vasoactive compounds.
author VINET,RAÚL
CORTÉS,MAGDALENA
LUXORO,MARIO
DELPIANO,MARCO A
author_facet VINET,RAÚL
CORTÉS,MAGDALENA
LUXORO,MARIO
DELPIANO,MARCO A
author_sort VINET,RAÚL
title Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
title_short Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
title_full Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
title_fullStr Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
title_full_unstemmed Nicotine-evoked cytosolic Ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
title_sort nicotine-evoked cytosolic ca2+ increase and cell depolarization in capillary endothelial cells of the bovine adrenal medulla
publisher Sociedad de Biología de Chile
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000100012
work_keys_str_mv AT vinetraul nicotineevokedcytosolicca2increaseandcelldepolarizationincapillaryendothelialcellsofthebovineadrenalmedulla
AT cortesmagdalena nicotineevokedcytosolicca2increaseandcelldepolarizationincapillaryendothelialcellsofthebovineadrenalmedulla
AT luxoromario nicotineevokedcytosolicca2increaseandcelldepolarizationincapillaryendothelialcellsofthebovineadrenalmedulla
AT delpianomarcoa nicotineevokedcytosolicca2increaseandcelldepolarizationincapillaryendothelialcellsofthebovineadrenalmedulla
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