Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies

DNA extraction methods for genotyping non-invasive samples have led to great advances in molecular research for ecological studies, and have been particularly useful for analyzing threatened species. However, scarce amounts of fragmented DNA and the presence of Taq polymerase inhibitors in non-invas...

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Autores principales:	MARRERO,PATRICIA, FREGEL,ROSA, CABRERA,VICENTE M, NOGALES,MANUEL
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	DNA extraction ecological studies guanidine thiocyanate non-invasive method pigeons seed dispersal
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200002
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spelling	oai:scielo:S0716-976020090002000022009-10-09Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studiesMARRERO,PATRICIAFREGEL,ROSACABRERA,VICENTE MNOGALES,MANUEL DNA extraction ecological studies guanidine thiocyanate non-invasive method pigeons seed dispersal DNA extraction methods for genotyping non-invasive samples have led to great advances in molecular research for ecological studies, and have been particularly useful for analyzing threatened species. However, scarce amounts of fragmented DNA and the presence of Taq polymerase inhibitors in non-invasive samples are potential problems for subsequent PCR amplifications. In this study we describe a novel technique for extracting DNA from alimentary tract cells found on external surfaces of feces and regurgitated seeds. The presence of contaminants and inhibitors is minimized and samples are preserved intact for use in other ecological research (e.g. trophic studies). The amplification efficiency and purity of the extracted DNA from feces were significantly higher than in commonly used extraction procedures. Moreover, DNA of two bird species was identified from seeds expelled by regurgitation. Therefore, this method may be suitable for future ecological studies of birds, and other vertebrate groups.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.2 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200002en10.4067/S0716-97602009000200002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	DNA extraction ecological studies guanidine thiocyanate non-invasive method pigeons seed dispersal
spellingShingle	DNA extraction ecological studies guanidine thiocyanate non-invasive method pigeons seed dispersal MARRERO,PATRICIA FREGEL,ROSA CABRERA,VICENTE M NOGALES,MANUEL Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
description	DNA extraction methods for genotyping non-invasive samples have led to great advances in molecular research for ecological studies, and have been particularly useful for analyzing threatened species. However, scarce amounts of fragmented DNA and the presence of Taq polymerase inhibitors in non-invasive samples are potential problems for subsequent PCR amplifications. In this study we describe a novel technique for extracting DNA from alimentary tract cells found on external surfaces of feces and regurgitated seeds. The presence of contaminants and inhibitors is minimized and samples are preserved intact for use in other ecological research (e.g. trophic studies). The amplification efficiency and purity of the extracted DNA from feces were significantly higher than in commonly used extraction procedures. Moreover, DNA of two bird species was identified from seeds expelled by regurgitation. Therefore, this method may be suitable for future ecological studies of birds, and other vertebrate groups.
author	MARRERO,PATRICIA FREGEL,ROSA CABRERA,VICENTE M NOGALES,MANUEL
author_facet	MARRERO,PATRICIA FREGEL,ROSA CABRERA,VICENTE M NOGALES,MANUEL
author_sort	MARRERO,PATRICIA
title	Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
title_short	Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
title_full	Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
title_fullStr	Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
title_full_unstemmed	Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
title_sort	extraction of high-quality host dna from feces and regurgitated seeds: a useful tool for vertebrate ecological studies
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200002
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Extraction of high-quality host DNA from feces and regurgitated seeds: a useful tool for vertebrate ecological studies

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