The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L

The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L

Hyptidendron canum (Pohl ex Benth.) Harley is a native tree of the Brazilian Savannah. The fish Oreochromis niloticus L. was used as an experimental model to determine the bioactivity of the crude ethanol extract as well as ethyl acetate, hexanic and chloroform fractions obtained from its leaves. Th...

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Autores principales: FIUZA,TATIANA S, SILVA,PAULO C, PAULA,JOSÉ R, TRESVENZOL,LEONICE M F, SABÓIA-MORAIS,SIMONE M T
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2009
Materias:
fish
Nile tilapia
Savannah biome
vasodilation
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200003
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spelling oai:scielo:S0716-976020090002000032009-10-09The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus LFIUZA,TATIANA SSILVA,PAULO CPAULA,JOSÉ RTRESVENZOL,LEONICE M FSABÓIA-MORAIS,SIMONE M T fish Nile tilapia Savannah biome vasodilation Hyptidendron canum (Pohl ex Benth.) Harley is a native tree of the Brazilian Savannah. The fish Oreochromis niloticus L. was used as an experimental model to determine the bioactivity of the crude ethanol extract as well as ethyl acetate, hexanic and chloroform fractions obtained from its leaves. The plant ethanol extract and fractions were administered to the fish orally with their feed. Twenty four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that both the crude ethanol extract and the fractions from H. canum induced vasoactive activity, causing vasodilation and vascular congestion, and the hexanic fraction also caused an apparent proliferation of capillaries. Hepatopancreas toxicity was evident through inflammatory processes. Pancreatic (chloroform fraction) and hepatic alterations, hemorrhagic spots and necroses were observed in fish treated with-ethanol extract and fractions. This study is the first description of the biologic action of the crude ethanol extract and the hexane, ethyl acetate and chloroform fractions in fish.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.2 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200003en10.4067/S0716-97602009000200003
institution Scielo Chile
collection Scielo Chile
language English
topic fish
Nile tilapia
Savannah biome
vasodilation
spellingShingle fish
Nile tilapia
Savannah biome
vasodilation
FIUZA,TATIANA S
SILVA,PAULO C
PAULA,JOSÉ R
TRESVENZOL,LEONICE M F
SABÓIA-MORAIS,SIMONE M T
The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
description Hyptidendron canum (Pohl ex Benth.) Harley is a native tree of the Brazilian Savannah. The fish Oreochromis niloticus L. was used as an experimental model to determine the bioactivity of the crude ethanol extract as well as ethyl acetate, hexanic and chloroform fractions obtained from its leaves. The plant ethanol extract and fractions were administered to the fish orally with their feed. Twenty four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that both the crude ethanol extract and the fractions from H. canum induced vasoactive activity, causing vasodilation and vascular congestion, and the hexanic fraction also caused an apparent proliferation of capillaries. Hepatopancreas toxicity was evident through inflammatory processes. Pancreatic (chloroform fraction) and hepatic alterations, hemorrhagic spots and necroses were observed in fish treated with-ethanol extract and fractions. This study is the first description of the biologic action of the crude ethanol extract and the hexane, ethyl acetate and chloroform fractions in fish.
author FIUZA,TATIANA S
SILVA,PAULO C
PAULA,JOSÉ R
TRESVENZOL,LEONICE M F
SABÓIA-MORAIS,SIMONE M T
author_facet FIUZA,TATIANA S
SILVA,PAULO C
PAULA,JOSÉ R
TRESVENZOL,LEONICE M F
SABÓIA-MORAIS,SIMONE M T
author_sort FIUZA,TATIANA S
title The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
title_short The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
title_full The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
title_fullStr The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
title_full_unstemmed The effect of crude ethanol extract and fractions of Hyptidendron canum (Pohl ex Benth.) Harley on the hepatopancreas of Oreochromis niloticus L
title_sort effect of crude ethanol extract and fractions of hyptidendron canum (pohl ex benth.) harley on the hepatopancreas of oreochromis niloticus l
publisher Sociedad de Biología de Chile
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200003
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