Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride

Stannous chloride (SnC12) is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of...

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Autores principales:	SOUZA,RAPHAEL S S, ALMEIDA,MARCELA C, MANOEL,CRISTIANO V, SANTOS-FILHO,SEBASTIÃO D, FONSECA,ADENILSON S, FILHO,MARIO BERNARDO
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	Escherichia coli free radicals Salix alba stannous chloride
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200008
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spelling	oai:scielo:S0716-976020090002000082009-10-09Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chlorideSOUZA,RAPHAEL S SALMEIDA,MARCELA CMANOEL,CRISTIANO VSANTOS-FILHO,SEBASTIÃO DFONSECA,ADENILSON SFILHO,MARIO BERNARDO Escherichia coli free radicals Salix alba stannous chloride Stannous chloride (SnC12) is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli (E. coli) AB1157 (wild type) cultures submitted to the action of SnC12. E. coli AB1157 cultures (exponential growth phase) were collected by centrifugation, washed and resuspended in 0.9%NaCl. Samples were incubated in water bath shaker with: (a) SnC12 (25mg/ml), (b)Salix alba extract(11.6mg/ml) and (c)SnC12(25mg/ml) + Salix alba extract (11.6mg/ml). Incubation with 0.9% NaCl was also carried out (control). At 60 min intervals, aliquots were withdrawn, diluted, spread onto Petri dishes with solid LB medium and incubated overnight. The colonies formed were counted and the survival fractions calculated. The extract was not able to protect the E. coli cultures against the lesive action of SnC12. The extract also did not interfere with the survival of the cultures. It suggested that the substances present in the Salix alba aqueous extract did not interfere strongly with cellular metabolism and did not alter the survival fractions of E. coli AB 1157. It is speculated that this extract cannot interfere with the generation of free radicals, the possible main agent responsible for SnC12 lesive action.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.2 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200008en10.4067/S0716-97602009000200008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Escherichia coli free radicals Salix alba stannous chloride
spellingShingle	Escherichia coli free radicals Salix alba stannous chloride SOUZA,RAPHAEL S S ALMEIDA,MARCELA C MANOEL,CRISTIANO V SANTOS-FILHO,SEBASTIÃO D FONSECA,ADENILSON S FILHO,MARIO BERNARDO Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
description	Stannous chloride (SnC12) is used in nuclear medicine as a reducing agent to obtain technetium-99m-radiopharmaceuticals. It have been reported that natural products might reduce the genotoxic and cytotoxic effects related to SnC12. This work evaluated the biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli (E. coli) AB1157 (wild type) cultures submitted to the action of SnC12. E. coli AB1157 cultures (exponential growth phase) were collected by centrifugation, washed and resuspended in 0.9%NaCl. Samples were incubated in water bath shaker with: (a) SnC12 (25mg/ml), (b)Salix alba extract(11.6mg/ml) and (c)SnC12(25mg/ml) + Salix alba extract (11.6mg/ml). Incubation with 0.9% NaCl was also carried out (control). At 60 min intervals, aliquots were withdrawn, diluted, spread onto Petri dishes with solid LB medium and incubated overnight. The colonies formed were counted and the survival fractions calculated. The extract was not able to protect the E. coli cultures against the lesive action of SnC12. The extract also did not interfere with the survival of the cultures. It suggested that the substances present in the Salix alba aqueous extract did not interfere strongly with cellular metabolism and did not alter the survival fractions of E. coli AB 1157. It is speculated that this extract cannot interfere with the generation of free radicals, the possible main agent responsible for SnC12 lesive action.
author	SOUZA,RAPHAEL S S ALMEIDA,MARCELA C MANOEL,CRISTIANO V SANTOS-FILHO,SEBASTIÃO D FONSECA,ADENILSON S FILHO,MARIO BERNARDO
author_facet	SOUZA,RAPHAEL S S ALMEIDA,MARCELA C MANOEL,CRISTIANO V SANTOS-FILHO,SEBASTIÃO D FONSECA,ADENILSON S FILHO,MARIO BERNARDO
author_sort	SOUZA,RAPHAEL S S
title	Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
title_short	Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
title_full	Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
title_fullStr	Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
title_full_unstemmed	Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride
title_sort	biological effects of an aqueous extract of salix alba on the survival of escherichia coli ab1157 cultures submitted to the action of stannous chloride
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200008
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Biological effects of an aqueous extract of Salix alba on the survival of Escherichia coli AB1157 cultures submitted to the action of stannous chloride

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