Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria

The natural resistance of plants to disease is based not only on preformed mechanisms, but also on induced mechanisms. The defense mechanisms present in resistant plants may also be found in susceptible ones. This study attempted to analyze the metabolic alterations in plants of the potato Solanum t...

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Autores principales:	POIATTI,VERA A D, DALMAS,FERNANDO R, ASTARITA,LEANDRO V
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	flavonoids hypersensitive response peroxidases phenolic compounds
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200009
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spelling	oai:scielo:S0716-976020090002000092009-10-09Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteriaPOIATTI,VERA A DDALMAS,FERNANDO RASTARITA,LEANDRO V flavonoids hypersensitive response peroxidases phenolic compounds The natural resistance of plants to disease is based not only on preformed mechanisms, but also on induced mechanisms. The defense mechanisms present in resistant plants may also be found in susceptible ones. This study attempted to analyze the metabolic alterations in plants of the potato Solanum tuberosum L. cv. Agata that were inoculated with the incompatible plant-pathogenic bacteria X. axonopodis and R. solanacearum, and the compatible bacterium E. carotovora. Levels of total phenolic compounds, including the flavonoid group, and the activities of polyphenol oxidase (PPO) and peroxidase (POX) were evaluated. Bacteria compatibility was evaluated by means of infiltration of tubers. The defense response was evaluated in the leaves of the potato plants. Leaves were inoculated depending on their number and location on the stem. Multiple-leaf inoculation was carried out on basal, intermediate, and apical leaves, and single inoculations on intermediate leaves. Leaves inoculated with X. axonopodis and with R. solanacearum showed hypersensitive responses within 24 hours post-inoculation, whereas leaves inoculated with E. carotovora showed disease symptoms. Therefore, the R. solanacearum isolate used in the experiments did not exhibit virulence to this potato cultivar. Regardless of the bacterial treatments, the basal leaves showed higher PPO and POX activities and lower levels of total phenolic compounds and flavonoids, compared to the apical leaves. However, basal and intermediate leaves inoculated with R. solanacearum and X. axonopodis showed increases in total phenolic compounds and flavonoid levels. In general, multiple-leaf inoculation showed the highest levels of total phenolics and flavonoids, whereas the single inoculations resulted in the highest increase in PPO activity. The POX activity showed no significant difference between single- and multiple-leaf inoculations. Plants inoculated with E. carotovora showed no significant increase in defense mechanisms such as enzyme activity and phenolic compounds. Therefore, resistance or susceptibility in S. tuberosum cv. Agata might depend on leaf age, type of inoculation performed (single or multiple), and the interaction between plant and pathogen.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.2 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200009en10.4067/S0716-97602009000200009
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	flavonoids hypersensitive response peroxidases phenolic compounds
spellingShingle	flavonoids hypersensitive response peroxidases phenolic compounds POIATTI,VERA A D DALMAS,FERNANDO R ASTARITA,LEANDRO V Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
description	The natural resistance of plants to disease is based not only on preformed mechanisms, but also on induced mechanisms. The defense mechanisms present in resistant plants may also be found in susceptible ones. This study attempted to analyze the metabolic alterations in plants of the potato Solanum tuberosum L. cv. Agata that were inoculated with the incompatible plant-pathogenic bacteria X. axonopodis and R. solanacearum, and the compatible bacterium E. carotovora. Levels of total phenolic compounds, including the flavonoid group, and the activities of polyphenol oxidase (PPO) and peroxidase (POX) were evaluated. Bacteria compatibility was evaluated by means of infiltration of tubers. The defense response was evaluated in the leaves of the potato plants. Leaves were inoculated depending on their number and location on the stem. Multiple-leaf inoculation was carried out on basal, intermediate, and apical leaves, and single inoculations on intermediate leaves. Leaves inoculated with X. axonopodis and with R. solanacearum showed hypersensitive responses within 24 hours post-inoculation, whereas leaves inoculated with E. carotovora showed disease symptoms. Therefore, the R. solanacearum isolate used in the experiments did not exhibit virulence to this potato cultivar. Regardless of the bacterial treatments, the basal leaves showed higher PPO and POX activities and lower levels of total phenolic compounds and flavonoids, compared to the apical leaves. However, basal and intermediate leaves inoculated with R. solanacearum and X. axonopodis showed increases in total phenolic compounds and flavonoid levels. In general, multiple-leaf inoculation showed the highest levels of total phenolics and flavonoids, whereas the single inoculations resulted in the highest increase in PPO activity. The POX activity showed no significant difference between single- and multiple-leaf inoculations. Plants inoculated with E. carotovora showed no significant increase in defense mechanisms such as enzyme activity and phenolic compounds. Therefore, resistance or susceptibility in S. tuberosum cv. Agata might depend on leaf age, type of inoculation performed (single or multiple), and the interaction between plant and pathogen.
author	POIATTI,VERA A D DALMAS,FERNANDO R ASTARITA,LEANDRO V
author_facet	POIATTI,VERA A D DALMAS,FERNANDO R ASTARITA,LEANDRO V
author_sort	POIATTI,VERA A D
title	Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
title_short	Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
title_full	Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
title_fullStr	Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
title_full_unstemmed	Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria
title_sort	defense mechanisms of solanum tuberosum l. in response to attack by plant-pathogenic bacteria
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200009
work_keys_str_mv	AT poiattiveraad defensemechanismsofsolanumtuberosumlinresponsetoattackbyplantpathogenicbacteria AT dalmasfernandor defensemechanismsofsolanumtuberosumlinresponsetoattackbyplantpathogenicbacteria AT astaritaleandrov defensemechanismsofsolanumtuberosumlinresponsetoattackbyplantpathogenicbacteria
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Defense mechanisms of Solanum tuberosum L. in response to attack by plant-pathogenic bacteria

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