Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer

Several studies revealed a similar down-regulation of telomeric repeat binding factor 1 (TRF1) in tumors. We have previously reported the TRFl expression levels were down-regulation in non-small cell lung cancer (NSCLC). The regulation of TRFl localization is proposed to be important for the functio...

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Autores principales:	JIAN,HU, LI,SUN, LU-MING,WANG, SU-JUN,JIANG
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	non-small cell lung cancer telomeric repeat binding factor 1 nuclear of localization signal nuclear of export signal DNA sequencing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200010
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spelling	oai:scielo:S0716-976020090002000102009-10-09Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancerJIAN,HULI,SUNLU-MING,WANGSU-JUN,JIANG non-small cell lung cancer telomeric repeat binding factor 1 nuclear of localization signal nuclear of export signal DNA sequencing Several studies revealed a similar down-regulation of telomeric repeat binding factor 1 (TRF1) in tumors. We have previously reported the TRFl expression levels were down-regulation in non-small cell lung cancer (NSCLC). The regulation of TRFl localization is proposed to be important for the function and expression. The nuclear localization signal (NLS) and nuclear export signal (NES) are often important clues to localization of protein. The objective of the present study was to investigate the NLS and NES of TRFl in NSCLC patients. Thirty (30) patients with NSCLCs had undergone radical operations in The First Affiliated Hospital, College of Medicine, Zhejiang University. DNA sequences of NLSs and NESs were amplified by PCR. The PCR products were analyzed by DNA sequencing. There were four NLSs of the TRFl protein, including two monopartite and two bipartite NLSs. The NLSs sequences were included in 337KKERRVGTPQSTKKKKESRR356. The exon 8 and exon 9 of TRFl DNA were covered the NLS sequences. The sequences of predicted NESs were 11WMLDFLCLSL86 and 174NLLKLQALAV183, respectively. The exon 1, exon 3 and exon 4 of TRFl were covered the NES sequences. In NSCLCs, there was no a mutation, deletion, or substitution in NLS and NES of TRFl. We conclude that the NLS and NES sequences in NSCLCs patients did not have mutations. Down-expression of TRFl does not indicate gene mutation of NLS and NES in NSCLCs.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.2 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200010en10.4067/S0716-97602009000200010
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	non-small cell lung cancer telomeric repeat binding factor 1 nuclear of localization signal nuclear of export signal DNA sequencing
spellingShingle	non-small cell lung cancer telomeric repeat binding factor 1 nuclear of localization signal nuclear of export signal DNA sequencing JIAN,HU LI,SUN LU-MING,WANG SU-JUN,JIANG Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer
description	Several studies revealed a similar down-regulation of telomeric repeat binding factor 1 (TRF1) in tumors. We have previously reported the TRFl expression levels were down-regulation in non-small cell lung cancer (NSCLC). The regulation of TRFl localization is proposed to be important for the function and expression. The nuclear localization signal (NLS) and nuclear export signal (NES) are often important clues to localization of protein. The objective of the present study was to investigate the NLS and NES of TRFl in NSCLC patients. Thirty (30) patients with NSCLCs had undergone radical operations in The First Affiliated Hospital, College of Medicine, Zhejiang University. DNA sequences of NLSs and NESs were amplified by PCR. The PCR products were analyzed by DNA sequencing. There were four NLSs of the TRFl protein, including two monopartite and two bipartite NLSs. The NLSs sequences were included in 337KKERRVGTPQSTKKKKESRR356. The exon 8 and exon 9 of TRFl DNA were covered the NLS sequences. The sequences of predicted NESs were 11WMLDFLCLSL86 and 174NLLKLQALAV183, respectively. The exon 1, exon 3 and exon 4 of TRFl were covered the NES sequences. In NSCLCs, there was no a mutation, deletion, or substitution in NLS and NES of TRFl. We conclude that the NLS and NES sequences in NSCLCs patients did not have mutations. Down-expression of TRFl does not indicate gene mutation of NLS and NES in NSCLCs.
author	JIAN,HU LI,SUN LU-MING,WANG SU-JUN,JIANG
author_facet	JIAN,HU LI,SUN LU-MING,WANG SU-JUN,JIANG
author_sort	JIAN,HU
title	Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer
title_short	Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer
title_full	Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer
title_fullStr	Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer
title_full_unstemmed	Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer
title_sort	analysis of the nuclear localization signal of trf1 in non-small cell lung cancer
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000200010
work_keys_str_mv	AT jianhu analysisofthenuclearlocalizationsignaloftrf1innonsmallcelllungcancer AT lisun analysisofthenuclearlocalizationsignaloftrf1innonsmallcelllungcancer AT lumingwang analysisofthenuclearlocalizationsignaloftrf1innonsmallcelllungcancer AT sujunjiang analysisofthenuclearlocalizationsignaloftrf1innonsmallcelllungcancer
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Analysis of the nuclear localization signal of TRF1 in non-small cell lung cancer

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