Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas

A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 μm in size)....

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Main Authors:	MISHRA,PANKAJ K, MISHRA,SMITA, BISHT,SHEKHAR C, SELVAKUMAR,G, KUNDU,S, BISHT,J. K, GUPTA,HARI SHANKAR
Language:	English
Published:	Sociedad de Biología de Chile 2009
Subjects:	Cold tolerant Pseudomonas lurida 16S rRNA gene Nutrient uptake Plant growth promotion
Online Access:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300005
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spelling	oai:scielo:S0716-976020090003000052009-11-02Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian HimalayasMISHRA,PANKAJ KMISHRA,SMITABISHT,SHEKHAR CSELVAKUMAR,GKUNDU,SBISHT,J. KGUPTA,HARI SHANKAR Cold tolerant Pseudomonas lurida 16S rRNA gene Nutrient uptake Plant growth promotion A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 μm in size). It grew at temperatures ranging from 4 to 30°C, with a growth optimum at 28°C. It exhibited tolerance to a wide pH range (5-10; optimum 8.0) and salt concentrations up to 6% (wt/vol). Although it was sensitive to Rifampicin (R 20 μg mi-1), Gentamicin (G 3 μg mi-1), and Streptomycin (S 5 μg mi-1), it showed resistance to higher concentrations of Ampicillin (A 500 μg mi-1), Penicillin (P 300 μg mi-1), Polymixin B sulphate (Pb 100 μg mi-1) and Chloramphenicol (C 200 μg mi-1). The 16S rRNA sequence analysis revealed maximum identity with Pseudomonas lurida. The bacterium produced indole Acetic Acid (IAA) and solubilizes phosphate at 4, 15 and 28°C. It also retained its ability to produce rhamnolipids and siderophores at 15°C. Seed bacterization with the isolate enhanced the germination, shoot and root lengths of thirty-day-old wheat seedlings by 19.2, 30.0 & 22.9% respectively, as compared to the un-inoculated controls.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300005en10.4067/S0716-97602009000300005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Cold tolerant Pseudomonas lurida 16S rRNA gene Nutrient uptake Plant growth promotion
spellingShingle	Cold tolerant Pseudomonas lurida 16S rRNA gene Nutrient uptake Plant growth promotion MISHRA,PANKAJ K MISHRA,SMITA BISHT,SHEKHAR C SELVAKUMAR,G KUNDU,S BISHT,J. K GUPTA,HARI SHANKAR Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
description	A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 μm in size). It grew at temperatures ranging from 4 to 30°C, with a growth optimum at 28°C. It exhibited tolerance to a wide pH range (5-10; optimum 8.0) and salt concentrations up to 6% (wt/vol). Although it was sensitive to Rifampicin (R 20 μg mi-1), Gentamicin (G 3 μg mi-1), and Streptomycin (S 5 μg mi-1), it showed resistance to higher concentrations of Ampicillin (A 500 μg mi-1), Penicillin (P 300 μg mi-1), Polymixin B sulphate (Pb 100 μg mi-1) and Chloramphenicol (C 200 μg mi-1). The 16S rRNA sequence analysis revealed maximum identity with Pseudomonas lurida. The bacterium produced indole Acetic Acid (IAA) and solubilizes phosphate at 4, 15 and 28°C. It also retained its ability to produce rhamnolipids and siderophores at 15°C. Seed bacterization with the isolate enhanced the germination, shoot and root lengths of thirty-day-old wheat seedlings by 19.2, 30.0 & 22.9% respectively, as compared to the un-inoculated controls.
author	MISHRA,PANKAJ K MISHRA,SMITA BISHT,SHEKHAR C SELVAKUMAR,G KUNDU,S BISHT,J. K GUPTA,HARI SHANKAR
author_facet	MISHRA,PANKAJ K MISHRA,SMITA BISHT,SHEKHAR C SELVAKUMAR,G KUNDU,S BISHT,J. K GUPTA,HARI SHANKAR
author_sort	MISHRA,PANKAJ K
title	Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
title_short	Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
title_full	Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
title_fullStr	Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
title_full_unstemmed	Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
title_sort	isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium pseudomonas sp. nars9 (mtcc9002) from the indian himalayas
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300005
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Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas

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