Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas
A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 μm in size)....
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Sociedad de Biología de Chile
2009
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oai:scielo:S0716-976020090003000052009-11-02Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian HimalayasMISHRA,PANKAJ KMISHRA,SMITABISHT,SHEKHAR CSELVAKUMAR,GKUNDU,SBISHT,J. KGUPTA,HARI SHANKAR Cold tolerant Pseudomonas lurida 16S rRNA gene Nutrient uptake Plant growth promotion A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 μm in size). It grew at temperatures ranging from 4 to 30°C, with a growth optimum at 28°C. It exhibited tolerance to a wide pH range (5-10; optimum 8.0) and salt concentrations up to 6% (wt/vol). Although it was sensitive to Rifampicin (R 20 μg mi-1), Gentamicin (G 3 μg mi-1), and Streptomycin (S 5 μg mi-1), it showed resistance to higher concentrations of Ampicillin (A 500 μg mi-1), Penicillin (P 300 μg mi-1), Polymixin B sulphate (Pb 100 μg mi-1) and Chloramphenicol (C 200 μg mi-1). The 16S rRNA sequence analysis revealed maximum identity with Pseudomonas lurida. The bacterium produced indole Acetic Acid (IAA) and solubilizes phosphate at 4, 15 and 28°C. It also retained its ability to produce rhamnolipids and siderophores at 15°C. Seed bacterization with the isolate enhanced the germination, shoot and root lengths of thirty-day-old wheat seedlings by 19.2, 30.0 & 22.9% respectively, as compared to the un-inoculated controls.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300005en10.4067/S0716-97602009000300005 |
institution |
Scielo Chile |
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Scielo Chile |
language |
English |
topic |
Cold tolerant Pseudomonas lurida 16S rRNA gene Nutrient uptake Plant growth promotion |
spellingShingle |
Cold tolerant Pseudomonas lurida 16S rRNA gene Nutrient uptake Plant growth promotion MISHRA,PANKAJ K MISHRA,SMITA BISHT,SHEKHAR C SELVAKUMAR,G KUNDU,S BISHT,J. K GUPTA,HARI SHANKAR Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas |
description |
A bacterium that grows and expresses plant growth promotion traits at 4°C was isolated from the rhizospheric soil of Amaranth, cultivated at a high altitude location in the North Western Indian Himalayas. The isolate was Gram negative and the cells appeared as rods (2.91 x 0.71 μm in size). It grew at temperatures ranging from 4 to 30°C, with a growth optimum at 28°C. It exhibited tolerance to a wide pH range (5-10; optimum 8.0) and salt concentrations up to 6% (wt/vol). Although it was sensitive to Rifampicin (R 20 μg mi-1), Gentamicin (G 3 μg mi-1), and Streptomycin (S 5 μg mi-1), it showed resistance to higher concentrations of Ampicillin (A 500 μg mi-1), Penicillin (P 300 μg mi-1), Polymixin B sulphate (Pb 100 μg mi-1) and Chloramphenicol (C 200 μg mi-1). The 16S rRNA sequence analysis revealed maximum identity with Pseudomonas lurida. The bacterium produced indole Acetic Acid (IAA) and solubilizes phosphate at 4, 15 and 28°C. It also retained its ability to produce rhamnolipids and siderophores at 15°C. Seed bacterization with the isolate enhanced the germination, shoot and root lengths of thirty-day-old wheat seedlings by 19.2, 30.0 & 22.9% respectively, as compared to the un-inoculated controls. |
author |
MISHRA,PANKAJ K MISHRA,SMITA BISHT,SHEKHAR C SELVAKUMAR,G KUNDU,S BISHT,J. K GUPTA,HARI SHANKAR |
author_facet |
MISHRA,PANKAJ K MISHRA,SMITA BISHT,SHEKHAR C SELVAKUMAR,G KUNDU,S BISHT,J. K GUPTA,HARI SHANKAR |
author_sort |
MISHRA,PANKAJ K |
title |
Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas |
title_short |
Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas |
title_full |
Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas |
title_fullStr |
Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas |
title_full_unstemmed |
Isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium Pseudomonas sp. NARs9 (MTCC9002) from the Indian Himalayas |
title_sort |
isolation, molecular characterization and growth-promotion activities of a cold tolerant bacterium pseudomonas sp. nars9 (mtcc9002) from the indian himalayas |
publisher |
Sociedad de Biología de Chile |
publishDate |
2009 |
url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300005 |
work_keys_str_mv |
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