Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria

The effect of combinations of the crude methanolic extract of the leaves of Helichrysum pedunculatum and eight first-line antibiotics were investigated by time kill assays against a panel of bacterial strains that have been implicated in wound infections. The plant extract showed appreciable antibac...

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Autores principales:	AIYEGORO,OLAYINKA A, AFOLAYAN,ANTHONY J, OKOH,ANTHONY I
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	Helichrysum pedunculatum time kill assay synergism
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300007
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spelling	oai:scielo:S0716-976020090003000072009-11-02Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteriaAIYEGORO,OLAYINKA AAFOLAYAN,ANTHONY JOKOH,ANTHONY I Helichrysum pedunculatum time kill assay synergism The effect of combinations of the crude methanolic extract of the leaves of Helichrysum pedunculatum and eight first-line antibiotics were investigated by time kill assays against a panel of bacterial strains that have been implicated in wound infections. The plant extract showed appreciable antibacterial activities against the test bacteria with zones of inhibition ranging between 18 and 27 mm, and minimum inhibitory concentrations (MICs) varying between 0.1 and 5.0 mg/ml. The MICs of the test antibiotics range between 0.001 and 0.412 mg/ml, and combination of the plant extract and the antibiotics resulted in reduction of bacterial counts by between 0 and 6.63 Log10 cfu/ml. At V2 MIC, 56.81% synergy; 43.19% indifference and no antagonism were observed, and at MIC levels, 55.68% synergy; 44.32% indifference and no antagonism were observed when the extracts were combined with eight different antibiotics. In all, 60% of the interactions were synergistic. All combination regimes on Staphylococcus aureus ATCC 6538 yielded no synergy, neither was antagonism detected in any of the assays. We propose that extracts of the leaves of Helichrysum pedunculatum could be of relevance in combination therapy and as a source of resistance modifying principies that could be useful as treatment options for persistent wound infections.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300007en10.4067/S0716-97602009000300007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Helichrysum pedunculatum time kill assay synergism
spellingShingle	Helichrysum pedunculatum time kill assay synergism AIYEGORO,OLAYINKA A AFOLAYAN,ANTHONY J OKOH,ANTHONY I Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
description	The effect of combinations of the crude methanolic extract of the leaves of Helichrysum pedunculatum and eight first-line antibiotics were investigated by time kill assays against a panel of bacterial strains that have been implicated in wound infections. The plant extract showed appreciable antibacterial activities against the test bacteria with zones of inhibition ranging between 18 and 27 mm, and minimum inhibitory concentrations (MICs) varying between 0.1 and 5.0 mg/ml. The MICs of the test antibiotics range between 0.001 and 0.412 mg/ml, and combination of the plant extract and the antibiotics resulted in reduction of bacterial counts by between 0 and 6.63 Log10 cfu/ml. At V2 MIC, 56.81% synergy; 43.19% indifference and no antagonism were observed, and at MIC levels, 55.68% synergy; 44.32% indifference and no antagonism were observed when the extracts were combined with eight different antibiotics. In all, 60% of the interactions were synergistic. All combination regimes on Staphylococcus aureus ATCC 6538 yielded no synergy, neither was antagonism detected in any of the assays. We propose that extracts of the leaves of Helichrysum pedunculatum could be of relevance in combination therapy and as a source of resistance modifying principies that could be useful as treatment options for persistent wound infections.
author	AIYEGORO,OLAYINKA A AFOLAYAN,ANTHONY J OKOH,ANTHONY I
author_facet	AIYEGORO,OLAYINKA A AFOLAYAN,ANTHONY J OKOH,ANTHONY I
author_sort	AIYEGORO,OLAYINKA A
title	Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
title_short	Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
title_full	Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
title_fullStr	Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
title_full_unstemmed	Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
title_sort	synergistic interaction of helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300007
work_keys_str_mv	AT aiyegoroolayinkaa synergisticinteractionofhelichrysumpedunculatumleafextractswithantibioticsagainstwoundinfectionassociatedbacteria AT afolayananthonyj synergisticinteractionofhelichrysumpedunculatumleafextractswithantibioticsagainstwoundinfectionassociatedbacteria AT okohanthonyi synergisticinteractionofhelichrysumpedunculatumleafextractswithantibioticsagainstwoundinfectionassociatedbacteria
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Synergistic interaction of Helichrysum pedunculatum leaf extracts with antibiotics against wound infection associated bacteria

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