Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)

We had recently reported antibacterial activity in the crude extract of the stem bark of Afzelia africana (Akinpelu et al., 2008). In this study, we assessed the biocidal and cell membrane disruption potentials of fractions obtained from the crude extract of the plant. The aqueous (AQ) and butanol (...

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Autores principales:	AKINPELU,DAVID A, AIYEGORO,AYOBAMI O, OKOH,ANTHONY I
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	Afzelia africana antibacteria phytochemistry killing rate protein leakage
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300008
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spelling	oai:scielo:S0716-976020090003000082009-11-02Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)AKINPELU,DAVID AAIYEGORO,AYOBAMI OOKOH,ANTHONY I Afzelia africana antibacteria phytochemistry killing rate protein leakage We had recently reported antibacterial activity in the crude extract of the stem bark of Afzelia africana (Akinpelu et al., 2008). In this study, we assessed the biocidal and cell membrane disruption potentials of fractions obtained from the crude extract of the plant. The aqueous (AQ) and butanol (BL) fractions exhibited appreciable antibacterial activities against the test bacteria. The minimum inhibitory concentrations of the AQ and BL fractions ranged between 0.313 and 2.5 mg/ml, while their minimum bactericidal concentrations varied between 0.625 and 5.0 mg/ml. Also, the AQ fraction killed about 95.8% of E. coli cells within 105 min at a concentration of 5 mg/ml, while about 99.1% of Bacillus pumilus cells were killed by this fraction at the same concentration and exposure time. A similar trend was observed for the BL fraction. At a concentration of 5 mg/ml, the butanol fraction leaked 9.8 μg/ml of proteins from E. coli cells within 3 h, while the aqueous fraction leaked 6.5 μg/ml of proteins from the same organisms at the same concentration and exposure time. We propose that the stem bark of Afzelia africana is a potential source of bioactive compounds of importance to the pharmaceutical industry.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300008en10.4067/S0716-97602009000300008
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Afzelia africana antibacteria phytochemistry killing rate protein leakage
spellingShingle	Afzelia africana antibacteria phytochemistry killing rate protein leakage AKINPELU,DAVID A AIYEGORO,AYOBAMI O OKOH,ANTHONY I Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
description	We had recently reported antibacterial activity in the crude extract of the stem bark of Afzelia africana (Akinpelu et al., 2008). In this study, we assessed the biocidal and cell membrane disruption potentials of fractions obtained from the crude extract of the plant. The aqueous (AQ) and butanol (BL) fractions exhibited appreciable antibacterial activities against the test bacteria. The minimum inhibitory concentrations of the AQ and BL fractions ranged between 0.313 and 2.5 mg/ml, while their minimum bactericidal concentrations varied between 0.625 and 5.0 mg/ml. Also, the AQ fraction killed about 95.8% of E. coli cells within 105 min at a concentration of 5 mg/ml, while about 99.1% of Bacillus pumilus cells were killed by this fraction at the same concentration and exposure time. A similar trend was observed for the BL fraction. At a concentration of 5 mg/ml, the butanol fraction leaked 9.8 μg/ml of proteins from E. coli cells within 3 h, while the aqueous fraction leaked 6.5 μg/ml of proteins from the same organisms at the same concentration and exposure time. We propose that the stem bark of Afzelia africana is a potential source of bioactive compounds of importance to the pharmaceutical industry.
author	AKINPELU,DAVID A AIYEGORO,AYOBAMI O OKOH,ANTHONY I
author_facet	AKINPELU,DAVID A AIYEGORO,AYOBAMI O OKOH,ANTHONY I
author_sort	AKINPELU,DAVID A
title	Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
title_short	Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
title_full	Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
title_fullStr	Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
title_full_unstemmed	Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)
title_sort	studies on the biocidal and cell membrane disruption potentials of stem bark extracts of afzelia africana (smith)
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300008
work_keys_str_mv	AT akinpeludavida studiesonthebiocidalandcellmembranedisruptionpotentialsofstembarkextractsofafzeliaafricanasmith AT aiyegoroayobamio studiesonthebiocidalandcellmembranedisruptionpotentialsofstembarkextractsofafzeliaafricanasmith AT okohanthonyi studiesonthebiocidalandcellmembranedisruptionpotentialsofstembarkextractsofafzeliaafricanasmith
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Studies on the biocidal and cell membrane disruption potentials of stem bark extracts of Afzelia africana (Smith)

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