A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line

Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in...

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Autores principales:	ARANDA,EVELYN, OWEN,GARETH I
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	angiogenesis EA.hy926 HUVEC endothelial formula in vitro assay matrigel
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300012
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spelling	oai:scielo:S0716-976020090003000122009-11-02A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell lineARANDA,EVELYNOWEN,GARETH I angiogenesis EA.hy926 HUVEC endothelial formula in vitro assay matrigel Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have the capacity to generate pro- or anti-angiogenic factors when given to non-endothelial cells, which we define herein as angiogenic potential. In conclusion, we propose a novel formula that we are confident accurately reflects the degree of in vitro angiogenesis allowing the quantification of prospective angiogenic compounds.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.3 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300012en10.4067/S0716-97602009000300012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	angiogenesis EA.hy926 HUVEC endothelial formula in vitro assay matrigel
spellingShingle	angiogenesis EA.hy926 HUVEC endothelial formula in vitro assay matrigel ARANDA,EVELYN OWEN,GARETH I A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line
description	Angiogenesis, the development of new capillary vessels, has a host of clinical manifestations. The identification of agents that increase or decrease angiogenesis is of great pharmaceutical interest. Classically, in vitro angiogenesis utilizes human umbilical vein endothelial cells (HUVEC) grown in matrigel. This valid and simple method has the drawbacks that each cell population is distinct and the constraint of obtaining primary source material. Herein we utilize the established EA.hy926 endothelial cell line as our model for in vitro angiogenesis and present a novel formula to quantify endothelial cell remodeling to identify pro- and anti-angiogenic agents. Furthermore, our technique details the procedures to identify and quantify compounds that have the capacity to generate pro- or anti-angiogenic factors when given to non-endothelial cells, which we define herein as angiogenic potential. In conclusion, we propose a novel formula that we are confident accurately reflects the degree of in vitro angiogenesis allowing the quantification of prospective angiogenic compounds.
author	ARANDA,EVELYN OWEN,GARETH I
author_facet	ARANDA,EVELYN OWEN,GARETH I
author_sort	ARANDA,EVELYN
title	A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line
title_short	A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line
title_full	A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line
title_fullStr	A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line
title_full_unstemmed	A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line
title_sort	semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the ea.hy926 endothelial cell line
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000300012
work_keys_str_mv	AT arandaevelyn asemiquantitativeassaytoscreenforangiogeniccompoundsandcompoundswithangiogenicpotentialusingtheeahy926endothelialcellline AT owengarethi asemiquantitativeassaytoscreenforangiogeniccompoundsandcompoundswithangiogenicpotentialusingtheeahy926endothelialcellline AT arandaevelyn semiquantitativeassaytoscreenforangiogeniccompoundsandcompoundswithangiogenicpotentialusingtheeahy926endothelialcellline AT owengarethi semiquantitativeassaytoscreenforangiogeniccompoundsandcompoundswithangiogenicpotentialusingtheeahy926endothelialcellline
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A semi-quantitative assay to screen for angiogenic compounds and compounds with angiogenic potential using the EA.hy926 endothelial cell line

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