Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L

Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L

This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish or...

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Autores principales: FIUZA,TATIANA S, SILVA,PAULO C, PAULA,JOSÉ R DE, TRESVENZOL,LEONICE M F, SABÓIA-MORAIS,SIMONE M T
Lenguaje:English
Publicado: Sociedad de Biología de Chile 2009
Materias:
fish
medicinal plants
vasodilation
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400002
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spelling oai:scielo:S0716-976020090004000022010-03-17Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus LFIUZA,TATIANA SSILVA,PAULO CPAULA,JOSÉ R DETRESVENZOL,LEONICE M FSABÓIA-MORAIS,SIMONE M T fish medicinal plants vasodilation This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish orally with their feed. Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude extract and the ethyl, chloroform and hexane fractions induced vasodilation, vascular congestion and toxicity due to the presence of eosinophilic granular cells, rodlet cells, some leukocytic infiltrate and rare focal necroses. The Nile tilapia proved to be a satisfactory model for screening plant products.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.4 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400002en10.4067/S0716-97602009000400002
institution Scielo Chile
collection Scielo Chile
language English
topic fish
medicinal plants
vasodilation
spellingShingle fish
medicinal plants
vasodilation
FIUZA,TATIANA S
SILVA,PAULO C
PAULA,JOSÉ R DE
TRESVENZOL,LEONICE M F
SABÓIA-MORAIS,SIMONE M T
Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
description This study evaluates the bioactivity of the crude ethanol extract and ethyl acetate, hexane and chloroform fractions obtained from Eugenia uniflora leaves using the hepatopancreas of Oreochromis niloticus L. as an experimental model. The ethanol extract and fractions were administered to the fish orally with their feed. Twenty-four hours later, the fish were sacrificed and their livers dissected, fixed in neutral formalin, embedded in paraffin and sectioned. Histological analyses were performed using Masson's trichrome and Haematoxylin-Eosin. Histochemical studies were performed using Feulgen, PAS (Periodic Acid Schiff) and PAS + salivary amylase and Sudan IV stain. The qualitative analysis of the material showed that the crude extract and the ethyl, chloroform and hexane fractions induced vasodilation, vascular congestion and toxicity due to the presence of eosinophilic granular cells, rodlet cells, some leukocytic infiltrate and rare focal necroses. The Nile tilapia proved to be a satisfactory model for screening plant products.
author FIUZA,TATIANA S
SILVA,PAULO C
PAULA,JOSÉ R DE
TRESVENZOL,LEONICE M F
SABÓIA-MORAIS,SIMONE M T
author_facet FIUZA,TATIANA S
SILVA,PAULO C
PAULA,JOSÉ R DE
TRESVENZOL,LEONICE M F
SABÓIA-MORAIS,SIMONE M T
author_sort FIUZA,TATIANA S
title Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
title_short Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
title_full Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
title_fullStr Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
title_full_unstemmed Bioactivity of crude ethanol extract and fractions of Eugenia uniflora (Myrtaceae) in the hepatopancreas of Oreochromis niloticus L
title_sort bioactivity of crude ethanol extract and fractions of eugenia uniflora (myrtaceae) in the hepatopancreas of oreochromis niloticus l
publisher Sociedad de Biología de Chile
publishDate 2009
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400002
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