Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes

Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activa...

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Autores principales:	ROJAS-DOTOR,SARA, PÉREZ-RAMOS,JULIA, GIMÉNEZ-SCHERER,JUAN ANTONIO, BLANCO-FAVELA,FRANCISCO, RICO-ROSILLO,GUADALUPE
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	Amebic hepatic abscess (AHA) CC chemokine receptors (CCR4, CCR5) monocyte locomotion inhibitory factor (MLIF) Entamoeba histolytica (E. histolytica) 1-phorbol-12 myristate-13 acetate (PMA) interleukin (IL) T helper 1 lymphocyte (Th1) T helper 2 lymphocyte Th2)
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003
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spelling	oai:scielo:S0716-976020090004000032010-03-17Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytesROJAS-DOTOR,SARAPÉREZ-RAMOS,JULIAGIMÉNEZ-SCHERER,JUAN ANTONIOBLANCO-FAVELA,FRANCISCORICO-ROSILLO,GUADALUPE Amebic hepatic abscess (AHA) CC chemokine receptors (CCR4, CCR5) monocyte locomotion inhibitory factor (MLIF) Entamoeba histolytica (E. histolytica) 1-phorbol-12 myristate-13 acetate (PMA) interleukin (IL) T helper 1 lymphocyte (Th1) T helper 2 lymphocyte Th2) Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-lß, IFN-γ, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-γ) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-γ/CCR5 and IL-1ß/CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.4 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003en10.4067/S0716-97602009000400003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Amebic hepatic abscess (AHA) CC chemokine receptors (CCR4, CCR5) monocyte locomotion inhibitory factor (MLIF) Entamoeba histolytica (E. histolytica) 1-phorbol-12 myristate-13 acetate (PMA) interleukin (IL) T helper 1 lymphocyte (Th1) T helper 2 lymphocyte Th2)
spellingShingle	Amebic hepatic abscess (AHA) CC chemokine receptors (CCR4, CCR5) monocyte locomotion inhibitory factor (MLIF) Entamoeba histolytica (E. histolytica) 1-phorbol-12 myristate-13 acetate (PMA) interleukin (IL) T helper 1 lymphocyte (Th1) T helper 2 lymphocyte Th2) ROJAS-DOTOR,SARA PÉREZ-RAMOS,JULIA GIMÉNEZ-SCHERER,JUAN ANTONIO BLANCO-FAVELA,FRANCISCO RICO-ROSILLO,GUADALUPE Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
description	Entamoeba histolytica produces Monocyte Locomotion Inhibitory Factor (MLIF), which may contribute to the delayed inflammation observed in amoebic hepatic abscesses. Leukocytes are affected through the modulation of cytokine expression and/or production. We evaluated the effects of MLIF on the activation and production of intracellular cytokines in human CD4+ T lymphocytes by flow cytometry. Cells were stimulated for 24 h with PMA, MLIF, or PMA+MLIF. Cellular activation was measured using anti-CD69. Th1/Th2 production was studied by the expression of intracellular cytokines and cytokine/chemokine receptors. MLIF increased CD69 and induced the over-expression of the IL-lß, IFN-γ, IL-2, IL-4, and IL-10 intracellular cytokines; PMA+MLIF inhibited Th1 cytokine (IFN-γ) and increased Th2 cytokines (IL-4 and IL-10). The co-expression of the cytokine and chemokine receptors IFN-γ/CCR5 and IL-1ß/CCR5 was inhibited by PMA+MLIF and Th2 co-expression was increased. MLIF effects varied depending on the conditions. MLIF alone activated the Th1 and Th2 cytokines and cytokine/receptor expression; however, PMA+MLIF increased the expression of Th2 but inhibited it in Th1.
author	ROJAS-DOTOR,SARA PÉREZ-RAMOS,JULIA GIMÉNEZ-SCHERER,JUAN ANTONIO BLANCO-FAVELA,FRANCISCO RICO-ROSILLO,GUADALUPE
author_facet	ROJAS-DOTOR,SARA PÉREZ-RAMOS,JULIA GIMÉNEZ-SCHERER,JUAN ANTONIO BLANCO-FAVELA,FRANCISCO RICO-ROSILLO,GUADALUPE
author_sort	ROJAS-DOTOR,SARA
title	Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
title_short	Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
title_full	Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
title_fullStr	Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
title_full_unstemmed	Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes
title_sort	effect of the monocyte locomotion inhibitory factor (mlif) produced by e. histolityca on cytokines and chemokine receptors in t cd4+ lymphocytes
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400003
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Effect of the monocyte locomotion inhibitory factor (MLIF) produced by E. histolityca on cytokines and chemokine receptors in T CD4+ lymphocytes

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