Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk

The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well d...

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Autores principales:	SOUZA VIANA,ELISETH, MARTINO CAMPOS,MARIA EMILENE, REIS PONCE,ADRIANA, CUQUETTO MANTOVANI,HILÁRIO, DANTAS VANETTI,MARIA CRISTINA
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	acyl homoserines lactones biofilm furanones quorum sensing
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400004
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spelling	oai:scielo:S0716-976020090004000042010-03-17Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milkSOUZA VIANA,ELISETHMARTINO CAMPOS,MARIA EMILENEREIS PONCE,ADRIANACUQUETTO MANTOVANI,HILÁRIODANTAS VANETTI,MARIA CRISTINA acyl homoserines lactones biofilm furanones quorum sensing The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy. The H. alvei 071 hall mutant was deficient in biofilm formation. All these results showed that the quorum sensing system is probably involved in the regulation of biofilm formation by H. alvei.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.4 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400004en10.4067/S0716-97602009000400004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	acyl homoserines lactones biofilm furanones quorum sensing
spellingShingle	acyl homoserines lactones biofilm furanones quorum sensing SOUZA VIANA,ELISETH MARTINO CAMPOS,MARIA EMILENE REIS PONCE,ADRIANA CUQUETTO MANTOVANI,HILÁRIO DANTAS VANETTI,MARIA CRISTINA Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
description	The objective of this study was to detect the presence of acyl homoserine lactones (AHLs), signal molecules of the quorum sensing system in biofilm formed by Hafnia alvei strains. It also evaluated the effect of synthetic quorum sensing inhibitors in biofilm formation. AHLs were assayed using well diffusion techniques, thin layer chromatography (TLC) and detection directly in biofilm with biomonitors. The extracts obtained from planktonic and sessile cell of H. alvei induced at least two of three monitor strains evaluated. The presence of AHLs with up to six carbon atoms was confirmed by TLC. Biofilm formation by H. alvei was inhibited by furanone, as demonstrated by 96-well assay of crystal violet in microtitre plates and by scanning electron microscopy. The H. alvei 071 hall mutant was deficient in biofilm formation. All these results showed that the quorum sensing system is probably involved in the regulation of biofilm formation by H. alvei.
author	SOUZA VIANA,ELISETH MARTINO CAMPOS,MARIA EMILENE REIS PONCE,ADRIANA CUQUETTO MANTOVANI,HILÁRIO DANTAS VANETTI,MARIA CRISTINA
author_facet	SOUZA VIANA,ELISETH MARTINO CAMPOS,MARIA EMILENE REIS PONCE,ADRIANA CUQUETTO MANTOVANI,HILÁRIO DANTAS VANETTI,MARIA CRISTINA
author_sort	SOUZA VIANA,ELISETH
title	Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
title_short	Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
title_full	Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
title_fullStr	Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
title_full_unstemmed	Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk
title_sort	biofilm formation and acyl homoserine lactone production in hafnia alvei isolated from raw milk
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400004
work_keys_str_mv	AT souzavianaeliseth biofilmformationandacylhomoserinelactoneproductioninhafniaalveiisolatedfromrawmilk AT martinocamposmariaemilene biofilmformationandacylhomoserinelactoneproductioninhafniaalveiisolatedfromrawmilk AT reisponceadriana biofilmformationandacylhomoserinelactoneproductioninhafniaalveiisolatedfromrawmilk AT cuquettomantovanihilario biofilmformationandacylhomoserinelactoneproductioninhafniaalveiisolatedfromrawmilk AT dantasvanettimariacristina biofilmformationandacylhomoserinelactoneproductioninhafniaalveiisolatedfromrawmilk
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Biofilm formation and acyl homoserine lactone production in Hafnia alvei isolated from raw milk

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