Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation

Calreticulin (CRT), a Ca2+-binding storage protein and chaperone in the endoplasmic reticulum, modulates cell adhesiveness and integrin-dependent Ca2+ signaling. However, the role of CRT during implantation remains poorly understood. In the present study, we characterized the expression of CRT mRNA...

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Autores principales:	CHENG,SHU-QUN, HE,JUN-LIN, DONG,YAN-LING, LIU,XUE-QING, DING,YU-BIN, GAO,RU-FEI, TAN,YI, YE,QIAN, TIAN,ZHEN-LING, WANG,YING-XIONG
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	Calreticulin Endometrium Embryo implantation Mouse
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400012
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spelling	oai:scielo:S0716-976020090004000122010-09-06Characterization of Calreticulin Expression in Mouse Endometrium during Embryo ImplantationCHENG,SHU-QUNHE,JUN-LINDONG,YAN-LINGLIU,XUE-QINGDING,YU-BINGAO,RU-FEITAN,YIYE,QIANTIAN,ZHEN-LINGWANG,YING-XIONG Calreticulin Endometrium Embryo implantation Mouse Calreticulin (CRT), a Ca2+-binding storage protein and chaperone in the endoplasmic reticulum, modulates cell adhesiveness and integrin-dependent Ca2+ signaling. However, the role of CRT during implantation remains poorly understood. In the present study, we characterized the expression of CRT mRNA and the protein in mouse endometria from pregnancy DI to D7. Real-Time PCR and in situ hybridization results showed that the levels of CRT mRNA in the endometria of pregnant mice were significantly higher than those of non-pregnant mice (P<0.05), and increased gradually from pregnancy DI to D4, reaching the máximum level on D4, followed by a plateau from D4 to D7. Using immunofluorescence histochemistry and western blot, changes of CRT expression in the endometria of pregnant mice were consistent with the expression of CRT mRNA. Furthermore, antisense CRT oligodeoxynucleotide was injected into the uterus horns of pregnant mice (D3) to investígate its effect on embryo implantation. The result showed that the number of implanted embryos markedly decreased in the side of uterine horns receiving antisense CRT oligodeoxynucleotide(í><0.05). These findings suggest that CRT may play an important role in embryo implantation in mice.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.4 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400012en10.4067/S0716-97602009000400012
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Calreticulin Endometrium Embryo implantation Mouse
spellingShingle	Calreticulin Endometrium Embryo implantation Mouse CHENG,SHU-QUN HE,JUN-LIN DONG,YAN-LING LIU,XUE-QING DING,YU-BIN GAO,RU-FEI TAN,YI YE,QIAN TIAN,ZHEN-LING WANG,YING-XIONG Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
description	Calreticulin (CRT), a Ca2+-binding storage protein and chaperone in the endoplasmic reticulum, modulates cell adhesiveness and integrin-dependent Ca2+ signaling. However, the role of CRT during implantation remains poorly understood. In the present study, we characterized the expression of CRT mRNA and the protein in mouse endometria from pregnancy DI to D7. Real-Time PCR and in situ hybridization results showed that the levels of CRT mRNA in the endometria of pregnant mice were significantly higher than those of non-pregnant mice (P<0.05), and increased gradually from pregnancy DI to D4, reaching the máximum level on D4, followed by a plateau from D4 to D7. Using immunofluorescence histochemistry and western blot, changes of CRT expression in the endometria of pregnant mice were consistent with the expression of CRT mRNA. Furthermore, antisense CRT oligodeoxynucleotide was injected into the uterus horns of pregnant mice (D3) to investígate its effect on embryo implantation. The result showed that the number of implanted embryos markedly decreased in the side of uterine horns receiving antisense CRT oligodeoxynucleotide(í><0.05). These findings suggest that CRT may play an important role in embryo implantation in mice.
author	CHENG,SHU-QUN HE,JUN-LIN DONG,YAN-LING LIU,XUE-QING DING,YU-BIN GAO,RU-FEI TAN,YI YE,QIAN TIAN,ZHEN-LING WANG,YING-XIONG
author_facet	CHENG,SHU-QUN HE,JUN-LIN DONG,YAN-LING LIU,XUE-QING DING,YU-BIN GAO,RU-FEI TAN,YI YE,QIAN TIAN,ZHEN-LING WANG,YING-XIONG
author_sort	CHENG,SHU-QUN
title	Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
title_short	Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
title_full	Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
title_fullStr	Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
title_full_unstemmed	Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation
title_sort	characterization of calreticulin expression in mouse endometrium during embryo implantation
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400012
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_version_	1718441453781254144

Characterization of Calreticulin Expression in Mouse Endometrium during Embryo Implantation

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