Induction of programmed cell death by Prangos uloptera, a medicinal plant

Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inh...

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Autores principales:	ZAHRI,SABER, RAZAVI,SEYED MEHDI, NIRI,FARSHAD HASSANZADEH, MOHAMMADI,SARIEH
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2009
Materias:	Apoptosis cytotoxicity McCoy Prangos uloptera
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400013
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spelling	oai:scielo:S0716-976020090004000132010-03-17Induction of programmed cell death by Prangos uloptera, a medicinal plantZAHRI,SABERRAZAVI,SEYED MEHDINIRI,FARSHAD HASSANZADEHMOHAMMADI,SARIEH Apoptosis cytotoxicity McCoy Prangos uloptera Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50% inhibition concentration and 50% cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic blebbing. These findings were confirmed by DNA fragmentation analysis. The results demonstrated efficient induction of apoptosis by the plant extract in moderate concentrations, but administration of higher concentrations showed that the primary manner of cell death was necrosis.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.42 n.4 20092009-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400013en10.4067/S0716-97602009000400013
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Apoptosis cytotoxicity McCoy Prangos uloptera
spellingShingle	Apoptosis cytotoxicity McCoy Prangos uloptera ZAHRI,SABER RAZAVI,SEYED MEHDI NIRI,FARSHAD HASSANZADEH MOHAMMADI,SARIEH Induction of programmed cell death by Prangos uloptera, a medicinal plant
description	Inhibition of the cell growth or induction of cell death is the most promising area in cancer therapy. The induction of apoptosis by dichloromethane extract of Prangos uloptera was evaluated on the McCoy cell line. This plant's roots, aerial parts and fruit have medicinal value. Cell growth inhibitory and cell cytotoxicity effects of the extract were assayed by MTT and Trypan-blue tests, respectively. Morphological changes and DNA fragmentation were also evaluated. The viability tests showed 0.49 and 0.3 mg/ml as 50% inhibition concentration and 50% cytotoxicity concentration after 24 hours of treatment, respectively. Fluorescent microscopy analysis revealed chromatin fragmentation and scanning electron microscopy showed cell shrinkage and cytoplasmic blebbing. These findings were confirmed by DNA fragmentation analysis. The results demonstrated efficient induction of apoptosis by the plant extract in moderate concentrations, but administration of higher concentrations showed that the primary manner of cell death was necrosis.
author	ZAHRI,SABER RAZAVI,SEYED MEHDI NIRI,FARSHAD HASSANZADEH MOHAMMADI,SARIEH
author_facet	ZAHRI,SABER RAZAVI,SEYED MEHDI NIRI,FARSHAD HASSANZADEH MOHAMMADI,SARIEH
author_sort	ZAHRI,SABER
title	Induction of programmed cell death by Prangos uloptera, a medicinal plant
title_short	Induction of programmed cell death by Prangos uloptera, a medicinal plant
title_full	Induction of programmed cell death by Prangos uloptera, a medicinal plant
title_fullStr	Induction of programmed cell death by Prangos uloptera, a medicinal plant
title_full_unstemmed	Induction of programmed cell death by Prangos uloptera, a medicinal plant
title_sort	induction of programmed cell death by prangos uloptera, a medicinal plant
publisher	Sociedad de Biología de Chile
publishDate	2009
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602009000400013
work_keys_str_mv	AT zahrisaber inductionofprogrammedcelldeathbyprangosulopteraamedicinalplant AT razaviseyedmehdi inductionofprogrammedcelldeathbyprangosulopteraamedicinalplant AT nirifarshadhassanzadeh inductionofprogrammedcelldeathbyprangosulopteraamedicinalplant AT mohammadisarieh inductionofprogrammedcelldeathbyprangosulopteraamedicinalplant
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Induction of programmed cell death by Prangos uloptera, a medicinal plant

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