Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay

The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the...

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Autores principales:	CALÁBRIA,LUCIANA KAREN, TEIXEIRA,RENATA ROLAND, COELHO GONÇALVES,SYBELLI MAGDA, PASSOS LIMA,ANDREIA BARCELOS, SANTOS,ANA ALICE DINIZ DOS, MARTINS,ANTÔNIO ROBERTO, ESPINDOLA,FOUED SALMEN
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	antigen retrieval microwave oven steam cooker
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002
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spelling	oai:scielo:S0716-976020100001000022010-05-07Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assayCALÁBRIA,LUCIANA KARENTEIXEIRA,RENATA ROLANDCOELHO GONÇALVES,SYBELLI MAGDAPASSOS LIMA,ANDREIA BARCELOSSANTOS,ANA ALICE DINIZ DOSMARTINS,ANTÔNIO ROBERTOESPINDOLA,FOUED SALMEN antigen retrieval microwave oven steam cooker The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002en10.4067/S0716-97602010000100002
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	antigen retrieval microwave oven steam cooker
spellingShingle	antigen retrieval microwave oven steam cooker CALÁBRIA,LUCIANA KAREN TEIXEIRA,RENATA ROLAND COELHO GONÇALVES,SYBELLI MAGDA PASSOS LIMA,ANDREIA BARCELOS SANTOS,ANA ALICE DINIZ DOS MARTINS,ANTÔNIO ROBERTO ESPINDOLA,FOUED SALMEN Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
description	The present study compared two heating methods currently used for antigen retrieval (AR) immunostaining: the microwave oven and the steam cooker. Myosin-V, a molecular motor involved in vesicle transport, was used as a neuronal marker in honeybee Apis mellifera brains fixed in formalin. Overall, the steam cooker showed the most satisfactory AR results. At 100 ºC, tissue morphology was maintained and revealed epitope recovery, while evaporation of the AR solution was markedly reduced; this is important for stabilizing the sodium citrate molarity of the AR buffer and reducing background effects. Standardization of heat-mediated AR of formalin-fixed and paraffin-embedded tissue sections results in more reliable immunostaining of the honeybee brain.
author	CALÁBRIA,LUCIANA KAREN TEIXEIRA,RENATA ROLAND COELHO GONÇALVES,SYBELLI MAGDA PASSOS LIMA,ANDREIA BARCELOS SANTOS,ANA ALICE DINIZ DOS MARTINS,ANTÔNIO ROBERTO ESPINDOLA,FOUED SALMEN
author_facet	CALÁBRIA,LUCIANA KAREN TEIXEIRA,RENATA ROLAND COELHO GONÇALVES,SYBELLI MAGDA PASSOS LIMA,ANDREIA BARCELOS SANTOS,ANA ALICE DINIZ DOS MARTINS,ANTÔNIO ROBERTO ESPINDOLA,FOUED SALMEN
author_sort	CALÁBRIA,LUCIANA KAREN
title	Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
title_short	Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
title_full	Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
title_fullStr	Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
title_full_unstemmed	Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay
title_sort	comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee apis mellifera: myosin-v assay
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100002
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Comparative analysis of two immunohistochemical methods for antigen retrieval in the optical lobe of the honeybee Apis mellifera: Myosin-v assay

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