Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG

Mycobacterium bovis BCG has been proposed as an effective live vector for multivalent vaccines. The development of mycobacterial genetic systems to express foreign antigens and the adjuvanticity of BCG are the basis for the potential use of this attenuated mycobacterium as a recombinant vaccine vect...

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Autores principales:	SEIXAS,FABIANA K, BORSUK,SIBELE, FAGUNDES,MICHEL Q, HARTWIG,DAIANE D, SILVA,ÉVERTON F. DA, CERQUEIRA,GUSTAVO M, DELLAGOSTIN,ODIR A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	recombinant BCG Auxotrophic complementation foreign antigens
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100003
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spelling	oai:scielo:S0716-976020100001000032010-05-07Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCGSEIXAS,FABIANA KBORSUK,SIBELEFAGUNDES,MICHEL QHARTWIG,DAIANE DSILVA,ÉVERTON F. DACERQUEIRA,GUSTAVO MDELLAGOSTIN,ODIR A recombinant BCG Auxotrophic complementation foreign antigens Mycobacterium bovis BCG has been proposed as an effective live vector for multivalent vaccines. The development of mycobacterial genetic systems to express foreign antigens and the adjuvanticity of BCG are the basis for the potential use of this attenuated mycobacterium as a recombinant vaccine vector. Stable plasmid vectors without antibiotic resistance markers are needed for heterologous antigen expression in BCG. Our group recently described the construction of a BCG expression system using auxotrophic complementation as a selectable marker. In this work, LipL32 and LigAni antigens of Leptospira interrogans were cloned and expressed in M. bovis BCG Pasteur and in the auxotrophic M. bovis BCG ΔleuD strains under the control of the M. leprae 18kDa promoter. Stability of the plasmids during in vitro growth and after inoculation of the recombinant BCG strains in hamsters was compared. The auxotrophic complementation system was highly stable, even during in vivo growth, as the selective pressure was maintained, whereas the conventional vector was unstable in the absence of selective pressure. These results confirm the usefulness of the new expression system, which represents a huge improvement over previously described expression systems for the development of BCG into an effective vaccine vector.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100003en10.4067/S0716-97602010000100003
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	recombinant BCG Auxotrophic complementation foreign antigens
spellingShingle	recombinant BCG Auxotrophic complementation foreign antigens SEIXAS,FABIANA K BORSUK,SIBELE FAGUNDES,MICHEL Q HARTWIG,DAIANE D SILVA,ÉVERTON F. DA CERQUEIRA,GUSTAVO M DELLAGOSTIN,ODIR A Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG
description	Mycobacterium bovis BCG has been proposed as an effective live vector for multivalent vaccines. The development of mycobacterial genetic systems to express foreign antigens and the adjuvanticity of BCG are the basis for the potential use of this attenuated mycobacterium as a recombinant vaccine vector. Stable plasmid vectors without antibiotic resistance markers are needed for heterologous antigen expression in BCG. Our group recently described the construction of a BCG expression system using auxotrophic complementation as a selectable marker. In this work, LipL32 and LigAni antigens of Leptospira interrogans were cloned and expressed in M. bovis BCG Pasteur and in the auxotrophic M. bovis BCG ΔleuD strains under the control of the M. leprae 18kDa promoter. Stability of the plasmids during in vitro growth and after inoculation of the recombinant BCG strains in hamsters was compared. The auxotrophic complementation system was highly stable, even during in vivo growth, as the selective pressure was maintained, whereas the conventional vector was unstable in the absence of selective pressure. These results confirm the usefulness of the new expression system, which represents a huge improvement over previously described expression systems for the development of BCG into an effective vaccine vector.
author	SEIXAS,FABIANA K BORSUK,SIBELE FAGUNDES,MICHEL Q HARTWIG,DAIANE D SILVA,ÉVERTON F. DA CERQUEIRA,GUSTAVO M DELLAGOSTIN,ODIR A
author_facet	SEIXAS,FABIANA K BORSUK,SIBELE FAGUNDES,MICHEL Q HARTWIG,DAIANE D SILVA,ÉVERTON F. DA CERQUEIRA,GUSTAVO M DELLAGOSTIN,ODIR A
author_sort	SEIXAS,FABIANA K
title	Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG
title_short	Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG
title_full	Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG
title_fullStr	Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG
title_full_unstemmed	Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG
title_sort	stable expression of leptospira interrogans antigens in auxotrophic mycobacterium bovis bcg
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100003
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Stable expression of Leptospira interrogans antigens in auxotrophic Mycobacterium bovis BCG

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