Cytotoxic activity of some marine brown algae against cancer cell lines

The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf...

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Autores principales:	KHANAVI,MAHNAZ, NABAVI,MARYAM, SADATI,NARGESS, SHAMS ARDEKANI,MOHAMMADREZA, SOHRABIPOUR,JELVE, NABAVI,SEYED MOHAMMAD B, GHAELI,PADIDEH, OSTAD,SEYED NASSER
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	Colpomenia sinuosa Cystoseira myrica Cytotoxic activity MTT assay Persian Gulf Sargassum swartzii
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100005
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spelling	oai:scielo:S0716-976020100001000052010-05-07Cytotoxic activity of some marine brown algae against cancer cell linesKHANAVI,MAHNAZNABAVI,MARYAMSADATI,NARGESSSHAMS ARDEKANI,MOHAMMADREZASOHRABIPOUR,JELVENABAVI,SEYED MOHAMMAD BGHAELI,PADIDEHOSTAD,SEYED NASSER Colpomenia sinuosa Cystoseira myrica Cytotoxic activity MTT assay Persian Gulf Sargassum swartzii The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in different cell lines including HT-29, Caco-2, T47D, MDA-MB468 and NIH 3T3 cell lines by MTT and AnnexinV-PI assay. The hexan fraction of S. swartzii and C. myrica showed selective cytotoxicity against proliferation of Caco-2 cells (IC50<100 μg/ml) T47D cell line (IC50<100 μg/ml), respectively. S. swartzii and C. myrica were also observed for increasing apoptosis in Caco-2 and T47D cells. Total extract and fractions of C. sinuosa did not show any significant cytotoxicity against the studied cell lines. MDA-MB468 cells were more sensitive to C. myrica than was T47D (IC50 99.9±8.11 vs. 56.50± 0.88). This reflects an estrogen receptor independent mechanism for cytotoxicity of the extract. The IC50 of the hexan fraction of C. myrica on T47D parent cells was lower than it was on T47D-TR cells (IC50 99.9±8.11 vs. 143.15 ± 7.80). This finding suggests a role for the MDR-1 in the development of possible future tolerance to the extract.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100005en10.4067/S0716-97602010000100005
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Colpomenia sinuosa Cystoseira myrica Cytotoxic activity MTT assay Persian Gulf Sargassum swartzii
spellingShingle	Colpomenia sinuosa Cystoseira myrica Cytotoxic activity MTT assay Persian Gulf Sargassum swartzii KHANAVI,MAHNAZ NABAVI,MARYAM SADATI,NARGESS SHAMS ARDEKANI,MOHAMMADREZA SOHRABIPOUR,JELVE NABAVI,SEYED MOHAMMAD B GHAELI,PADIDEH OSTAD,SEYED NASSER Cytotoxic activity of some marine brown algae against cancer cell lines
description	The aim of this study was to investigate the in vitro cytotoxic activity of total extract of MeOH (70%) and partition fractions of hexan, chloroform (CHCL3), ethylacetate (EtOAc) and MeOH-H2O of brown algae species (Sargassum swartzii, Cystoseira myrica, Colpomenia sinuosa) found in the Persian Gulf against in different cell lines including HT-29, Caco-2, T47D, MDA-MB468 and NIH 3T3 cell lines by MTT and AnnexinV-PI assay. The hexan fraction of S. swartzii and C. myrica showed selective cytotoxicity against proliferation of Caco-2 cells (IC50<100 μg/ml) T47D cell line (IC50<100 μg/ml), respectively. S. swartzii and C. myrica were also observed for increasing apoptosis in Caco-2 and T47D cells. Total extract and fractions of C. sinuosa did not show any significant cytotoxicity against the studied cell lines. MDA-MB468 cells were more sensitive to C. myrica than was T47D (IC50 99.9±8.11 vs. 56.50± 0.88). This reflects an estrogen receptor independent mechanism for cytotoxicity of the extract. The IC50 of the hexan fraction of C. myrica on T47D parent cells was lower than it was on T47D-TR cells (IC50 99.9±8.11 vs. 143.15 ± 7.80). This finding suggests a role for the MDR-1 in the development of possible future tolerance to the extract.
author	KHANAVI,MAHNAZ NABAVI,MARYAM SADATI,NARGESS SHAMS ARDEKANI,MOHAMMADREZA SOHRABIPOUR,JELVE NABAVI,SEYED MOHAMMAD B GHAELI,PADIDEH OSTAD,SEYED NASSER
author_facet	KHANAVI,MAHNAZ NABAVI,MARYAM SADATI,NARGESS SHAMS ARDEKANI,MOHAMMADREZA SOHRABIPOUR,JELVE NABAVI,SEYED MOHAMMAD B GHAELI,PADIDEH OSTAD,SEYED NASSER
author_sort	KHANAVI,MAHNAZ
title	Cytotoxic activity of some marine brown algae against cancer cell lines
title_short	Cytotoxic activity of some marine brown algae against cancer cell lines
title_full	Cytotoxic activity of some marine brown algae against cancer cell lines
title_fullStr	Cytotoxic activity of some marine brown algae against cancer cell lines
title_full_unstemmed	Cytotoxic activity of some marine brown algae against cancer cell lines
title_sort	cytotoxic activity of some marine brown algae against cancer cell lines
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100005
work_keys_str_mv	AT khanavimahnaz cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines AT nabavimaryam cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines AT sadatinargess cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines AT shamsardekanimohammadreza cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines AT sohrabipourjelve cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines AT nabaviseyedmohammadb cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines AT ghaelipadideh cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines AT ostadseyednasser cytotoxicactivityofsomemarinebrownalgaeagainstcancercelllines
_version_	1718441455234580480

Cytotoxic activity of some marine brown algae against cancer cell lines

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