Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro

In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo indu...

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Autores principales:	GARCÍA,KATHERINE P, RUBILAR,PAULINA S, VARGAS,MACARENA F, CÁRDENAS,HUGO, RIOS,MIGUEL A, ORIHUELA,PEDRO A, VARGAS,RENATO H , FUHRER,JUAN, HECKELS,JOHN E, CHRISTODOULIDES,MYRON, VELÁSQUEZ,LUIS A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	cellular damage fallopian tubes Neisseria gonorrhoeae nitric oxide
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100006
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spelling	oai:scielo:S0716-976020100001000062010-05-07Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitroGARCÍA,KATHERINE PRUBILAR,PAULINA SVARGAS,MACARENA FCÁRDENAS,HUGORIOS,MIGUEL AORIHUELA,PEDRO AVARGAS,RENATO H FUHRER,JUANHECKELS,JOHN ECHRISTODOULIDES,MYRONVELÁSQUEZ,LUIS A cellular damage fallopian tubes Neisseria gonorrhoeae nitric oxide In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS. Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release, which correlated with a ~50% reduction in gonococcal numbers. Moreover, treatment of normal FT explants with an exogenous NO donor, SNAP, did not induce significant cellular damage. Taken together, our data suggest that NO does not contribute to cellular damage during infection of the human FT with Neisseria gonorrhoeae.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100006en10.4067/S0716-97602010000100006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	cellular damage fallopian tubes Neisseria gonorrhoeae nitric oxide
spellingShingle	cellular damage fallopian tubes Neisseria gonorrhoeae nitric oxide GARCÍA,KATHERINE P RUBILAR,PAULINA S VARGAS,MACARENA F CÁRDENAS,HUGO RIOS,MIGUEL A ORIHUELA,PEDRO A VARGAS,RENATO H FUHRER,JUAN HECKELS,JOHN E CHRISTODOULIDES,MYRON VELÁSQUEZ,LUIS A Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
description	In the present study, we investigated whether cellular damage, as demonstrated by lactate dehydrogenase (LDH) release in the human fallopian tube (FT) infected by Neisseria gonorrhoeae (Ngo), correlated with high levels of nitric oxide synthase (NOS) mRNA and enzyme activity. Infection with Ngo induced a significant increase (~35-fold) in mRNA transcripts of the inducible isoform of NOS. Paradoxically, a reduction in NOS enzyme activity was observed in infected cultures, suggesting that gonococcal infection possibly influences translation of iNOS mRNA to the enzyme. In addition, treatment with the NOS inhibitor TRIM did not prevent gonococcal-induced cellular damage. In contrast, the addition of the inhibitor L-NAME induced a 40% reduction in LDH release, which correlated with a ~50% reduction in gonococcal numbers. Moreover, treatment of normal FT explants with an exogenous NO donor, SNAP, did not induce significant cellular damage. Taken together, our data suggest that NO does not contribute to cellular damage during infection of the human FT with Neisseria gonorrhoeae.
author	GARCÍA,KATHERINE P RUBILAR,PAULINA S VARGAS,MACARENA F CÁRDENAS,HUGO RIOS,MIGUEL A ORIHUELA,PEDRO A VARGAS,RENATO H FUHRER,JUAN HECKELS,JOHN E CHRISTODOULIDES,MYRON VELÁSQUEZ,LUIS A
author_facet	GARCÍA,KATHERINE P RUBILAR,PAULINA S VARGAS,MACARENA F CÁRDENAS,HUGO RIOS,MIGUEL A ORIHUELA,PEDRO A VARGAS,RENATO H FUHRER,JUAN HECKELS,JOHN E CHRISTODOULIDES,MYRON VELÁSQUEZ,LUIS A
author_sort	GARCÍA,KATHERINE P
title	Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
title_short	Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
title_full	Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
title_fullStr	Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
title_full_unstemmed	Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro
title_sort	nitric oxide is not involved in neisseria gonorrhoeae-induced cellular damage of human fallopian tubes in vitro
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100006
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Nitric oxide is not involved in Neisseria gonorrhoeae-induced cellular damage of human Fallopian tubes in vitro

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