Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis

The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profil...

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Autores principales:	SANTANNA,JULIANE R, MIYAMOTO,CLÁUDIA T, ROSADA,LÚCIA J, FRANCO,CLAUDINÉIA C S, KANESHIMA,EDILSON N, CASTRO-PRADO,MARIALBA A A
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2010
Materias:	genetic variability heterokaryosis parasexual recombination RAPD
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100007
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spelling	oai:scielo:S0716-976020100001000072010-05-07Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysisSANTANNA,JULIANE RMIYAMOTO,CLÁUDIA TROSADA,LÚCIA JFRANCO,CLAUDINÉIA C SKANESHIMA,EDILSON NCASTRO-PRADO,MARIALBA A A genetic variability heterokaryosis parasexual recombination RAPD The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profile. Based on pairings of nit mutants from the different isolates, five vegetative complementation groups (VCG) were identified, and barriers to the formation of heterokaryons were observed among isolates derived from the same geographic area. No complementation was observed among any of the nit mutants recovered from the isolate A, which was designed heterokaryon-self-incompatible. Based on RAPD analysis, a polymorphism was detected among the wild isolate C and their nit1 and NitM mutants. RAPD amplification, with five different primers, also showed polymorphic profiles among Brazilian C. truncatum isolates. Dendrogram analysis resulted in a similarity degree ranging between 0.331 and 0.882 among isolates and identified three RAPD groups. Despite the lack of a correlation between the RAPD analysis and the vegetative compatibility grouping, results demonstrated the potential of VCG analysis to differentiate C. truncatum isolates genotypically similar when compared by RAPD.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.43 n.1 20102010-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100007en10.4067/S0716-97602010000100007
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	genetic variability heterokaryosis parasexual recombination RAPD
spellingShingle	genetic variability heterokaryosis parasexual recombination RAPD SANTANNA,JULIANE R MIYAMOTO,CLÁUDIA T ROSADA,LÚCIA J FRANCO,CLAUDINÉIA C S KANESHIMA,EDILSON N CASTRO-PRADO,MARIALBA A A Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
description	The genetic variation among nine soybean-originating isolates of Colletotrichum truncatum from different Brazilian states was studied. Nitrate non-utilizing (nit) mutants were obtained with potassium chlorate and used to characterize vegetative compatibility reactions, heterokaryosis and RAPD profile. Based on pairings of nit mutants from the different isolates, five vegetative complementation groups (VCG) were identified, and barriers to the formation of heterokaryons were observed among isolates derived from the same geographic area. No complementation was observed among any of the nit mutants recovered from the isolate A, which was designed heterokaryon-self-incompatible. Based on RAPD analysis, a polymorphism was detected among the wild isolate C and their nit1 and NitM mutants. RAPD amplification, with five different primers, also showed polymorphic profiles among Brazilian C. truncatum isolates. Dendrogram analysis resulted in a similarity degree ranging between 0.331 and 0.882 among isolates and identified three RAPD groups. Despite the lack of a correlation between the RAPD analysis and the vegetative compatibility grouping, results demonstrated the potential of VCG analysis to differentiate C. truncatum isolates genotypically similar when compared by RAPD.
author	SANTANNA,JULIANE R MIYAMOTO,CLÁUDIA T ROSADA,LÚCIA J FRANCO,CLAUDINÉIA C S KANESHIMA,EDILSON N CASTRO-PRADO,MARIALBA A A
author_facet	SANTANNA,JULIANE R MIYAMOTO,CLÁUDIA T ROSADA,LÚCIA J FRANCO,CLAUDINÉIA C S KANESHIMA,EDILSON N CASTRO-PRADO,MARIALBA A A
author_sort	SANTANNA,JULIANE R
title	Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
title_short	Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
title_full	Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
title_fullStr	Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
title_full_unstemmed	Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis
title_sort	genetic relatedness of brazilian colletotrichum truncatum isolates assessed by vegetative compatibility groups and rapd analysis
publisher	Sociedad de Biología de Chile
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000100007
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Genetic relatedness of Brazilian Colletotrichum truncatum isolates assessed by vegetative compatibility groups and RAPD analysis

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